摘要
目的研究独一味止血有效部位总环烯醚萜苷对大鼠各项血液凝集参数的影响,同时对其毒性进行初步研究。方法大鼠口服给药独一味水提取物HLRE(3g.kg-1)、总黄酮(P1,0.36g.kg-1)、总环烯醚萜苷(P2,0.99g.kg-1)、大极性部分(P3,1.65g.kg-1)和生理盐水(NS)14d,颈总动脉采血,测定凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)、凝血酶时间(TT)、纤维蛋白原含量(FIB);大鼠口服给药P2(1.4、0.7、0.35g.kg-1)、云南白药(BY,0.9g.kg-1),测定各项凝血参数;小鼠口服及腹腔注射给药不同剂量的P2,计算其最大耐受量(MTD)和半数致死量(LD50)。结果与NS对照组比较,独一味各类成分给药后大鼠TT分别缩短18.59%、—3.12%、24.11%和9.92%,FIB含量分别增加25.32%、8.67%、28.29%和5.36%,HLRE及P2组有显著性差异(P<0.05);P2不同剂量灌胃给药14d后,各组均能使大鼠TT缩短、FIB增加,有一定剂量依赖性,高剂量组与NS组和BY组比较,均有显著统计学差异(P<0.05);各组间PT无显著性差异,高剂量使APTT显著延长(P<0.05)。总环烯醚萜苷1次最大口服剂量(55g.kg-1)未见明显的毒副作用。腹腔给药LD50为7.834g.kg-1,MTD为5.31g.kg-1。结论独一味总环烯醚萜苷可以显著缩短大鼠TT并增加FIB含量,有较好的量效关系,是其止血活性部位,且小鼠口服基本无毒性,比水提取物使用更安全。
OBJECTIVE： To study the effect of the hematostatic effective components of Herba Lamiophlomis rotata on rat＇ s blood conglomeration parameters. The toxicity of these components was simultaneously studied.METHODS： Herba Lamiophlomis rotata water extract （HLRE） （3g·kg^-1）, Total Flavonoids（P1, 0.36g·kg^-1）, Total Iridoid Glycosides（P2, 0.99g·kg^-1）, Maximus Polarity Components（P3, 1.65g·kg^-1） and normal sodium NS were given orally in rats for 14 days. Blood samples were collected from common carotid artery; prothrombin time （PT）, activated partial thromboplastin time （APTT）, thrombin time （TT）and fibrinogen （FIB） were tested. Different oral doses of（P2 , 1.4,0.7,0.35 g·kg^-1） and Yunnan white powder （BY, 0.9g·kg^-1） were given to rats and conglomeration parameters were determined too. The maximum tolerable dose （MTD） and LD50 of mice were determined after different doses of P2 were given with oral administration and intraperitoneal injection. RESULTS： Compared to NS control group, each composition of Lamiophlomis rotata Kudo can shorten the TT by 18.59% ,-3.12%, 24.11% and 9.92% respectively, and increase the content of FIB by 25.32%, 8.67%, 28.29% and 5.36% respectively. There was obvious change in HLRE and P2（P 〈0.05） . After 14 days intragastric administration of different dosage P2, TT decreased and FIB increased. As compared to NS and BY, the high dose group had significantly change （P〈0.05）, meanwhile there was no obvious diffenrence in PT. High dosage can remarkedly increase APTT （P〈 0.05） . Given 55g·kg^-1 of P2 with oral administration, there was no significant toxicant and adverse reaction in mice. But ginven P2 with intraperitoneal injection, the MTD was 5.31g·kg^-1 and LD50 was 7.834g·kg^-1. CONCLUSIONS： The Total Iridoid Glycosides （P2） of Herba Lamiophlomis rotata can increase the contents of FIB and shorten TT value with a good dose-effect relationship. No visible toxicant and adverse reactions were observed in mice after oral administration. The Total Iridoid Glycosides is safer than water extract.
出处
《中国药房》
CAS
CSCD
北大核心
2007年第3期231-233,共3页
China Pharmacy
基金
兰州军区医药卫生科研基金资助项目(LXH-200409)
甘肃省科技攻关项目(2GS054-A43-014-14)
关键词
独一味
总环烯醚萜苷
止血
有效部位
凝血参数
毒性
Herba Lamiophlomis Rotata.
Total
ridoid Glycosides
Heamostasis
Effective components
Conglomeration parameter
Toxicity
