摘要
目的建立草药半边旗中木犀草素含量的准确测定方法，并测定不同采收期半边旗中木犀草素的含量。方法采用高效液相色谱法，色谱柱为Diamonsil C18流动相为甲醇水梯度洗脱，流速0．2ml·min^-1，检测波长为283nm，柱温度35℃。结果木犀草素进样量在0．062-0．62μg范围内线性关系良好（R^2=0．9987），平均回收率为99．5％（RSD=1．98％）。结论该方法操作简便，结论可靠，可用于半边旗草药的质量控制。
Objective To establish an HPLC method for content determination of luteolin from PSL. Methods The separation was performed on a Diamonsil C18 column, the mobile phase was run with a mixture of methanol（B） and water（A） with a linear gradient elution at a flow rate of 0.2 ml · min , the ultraviolet wavelength was 283 nm, and the column temperature was maintained at 35℃. Results The calibration curve showed good linearity in the range of 0.062 - 0.62 μg（ R^2 = 0.998 7 ）, the average recovery was 99.5% （RSD = 1.98% ）. Conclusion The method is simple, convenient and accurate and can be used for the quality control of PSL.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2007年第1期102-103,共2页
Lishizhen Medicine and Materia Medica Research
