摘要
随着哺乳动物细胞培养规模的扩大和生物药物需求的增长,基于细胞及产品特性的无血清培养基的研制已成为细胞工程领域的重要课题。运用统计学实验方法,可科学有效地考察细胞培养基中多因素、多水平间的交互作用。应用新型蛋白质组分析技术及生物芯片技术定位胞浆信号通路相关蛋白、膜表面的生长因子受体、激素受体、细胞因子受体、粘附分子等,用于确定细胞培养基中调控分子的添加组合。系统概述了目前无血清培养基几种新型及常用的研究和设计方法,并对其应用特点作了分析,希望为动物细胞无血清培养基的研制提供可借鉴的思路。
With the expanding scale of animal cell culture and increasing demand of biopharmaceutical, the development of serum-free medium based on cell lines and products has become a major task in the field of cell engineer. The statistical methods and tools are popular in the processes of design medium and can be used to evaluate the multiple factors and their interaction scientifically and effectively. The novel microarray and proteomic analysis can improve the performance of identifying the role of medium. The aim is to provide some idea for serum-free medium study through systematically summarizing the newly and commonly used serum-free medium study and design approaches and their characteristics.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2007年第1期110-114,共5页
China Biotechnology
基金
国家"863"计划资助项目(2004A2Z3792)
