摘要
目的:探讨近交系大鼠原位肝移植模型的建立并判断排斥反应发生的强度.方法:采用Kamada二袖套法,利用封闭群大鼠SD和Wistar进行建模技能训练,在此基础上建立近交系大鼠DA→LEW肝移植模型,根据临床表现和Banff标准判断排斥反应发生的强度.结果:共施行DA→LEW大鼠肝移植模型15例,手术成功率86.7%,死亡原因为肝上下腔静脉漏血、肝下下腔静脉血栓、胆道梗阻.术后第3天开始出现Ⅰ级排斥反应,7d以后逐渐达到高峰,除术后并发症致死外,剩余均在12d内死于Ⅲ级排斥反应.结论:DA→LEW为稳定、强烈的大鼠肝移植急排模型,是研究肝移植排斥及免疫耐受的理想动物模型.但近交系大鼠在组织结构上有其自身特点,给建模带来一定难度.
AIM： To establish the orthotopic liver transplanta-ion models in inbred rats and judge the grades of acute rejection. METHODS： Surgical skills were trained by using closed colony SD and Wistar rats, followed by DA to LEW orthotopic liver transplantation with Kamada＇s two-cuff technique. The grades of acute rejection were judged by their clinical manifestations and Banff schema. RESULTS： Fifteen models of DA to LEW liver transplantation were established, the success rate being 86.7%. The causes of death were hemorrhage and thrombosis of suprahepatic and infrahepatic vena cava and obstruction of biliary tract. I grade rejection occurred at 3rd day and reached the peak at 7th day after transplantation. Remaining models died of m grade rejection besides of postoperative complication within 12 d after transplantation. CONCLUSION： The acute rejection of DA to LEW orthotopic liver transplantation model is stable and intensive. It can be effectively used to study allograft rejection and tolerance. But it is difficult to establish the kind of orthotopic liver transplantation models in inbred rats because of their own tissue characteristics.
出处
《第四军医大学学报》
CAS
北大核心
2007年第2期114-118,共5页
Journal of the Fourth Military Medical University
关键词
大鼠
近交系
肝移植
疾病模型
动物
移植物排斥
rats, inbred strains
liver transplantation
disease models, animal
graft rejection
