摘要
为研究全自养脱氮过程,采用斯凯尔曼亚硝化单胞杆菌培养基,从南京某城市污水处理厂序批式处理系统(SBR)中分离得到一株具有多途径氮代谢功能的荧光假单胞菌Pseudomonas fluorescensdN13。该菌株在有氧条件下,无有机碳源存在,能进行氧化NH3-N产能代谢生长;有有机碳源存在且NO2--N和NO3--N的浓度较高时,它能将NO2--N和NO3--N还原为NH3-N。在厌氧条件下,只有乙酸钠作碳源时,该菌株仍能进行硝酸亚硝酸还原(反硝化)作用。在这个具有多途径氮代谢功能的菌株细胞内,硝化反硝化过程耦合成为可能。
To study on autotrophie removal of nitrogen from wastewater containing a high concentration of NH3-N, a strain of Pseudomonasfluorescens dN13 was isolated, by means of incubation in Skerman＇ s medium for Nitrosomona, from the sequence batch reactor system（SBR）of a wastewater treatment plant in Nanjing. Under aerobic condition, this strain could oxidize NH3-N into NO2^- -N and grow without organic carbon source; and reduce NO2^- -N and NO3^- -N into NH3-N with organic carbon source and adequate concentration of NO2^- -N and NO3^- -N, while under anaerobic condition, strain dN13 could still reduce NO2^- -N and NO3 -N into NH3-N with sodium acetate as the sole carbon source. In the cells of strain dN13 with such multi-paths of nitrogen metabolism, coupling of nitrification and denitrification is feasible.
出处
《土壤学报》
CAS
CSCD
北大核心
2007年第1期144-149,共6页
Acta Pedologica Sinica
基金
江苏省自然科学基金项目(BK2002019)资助
关键词
荧光假单胞菌
有氧氨氧化
硝酸亚硝酸还原
多途径氮代谢
Pseudomonasfluorescens
Aerobic oxidation of ammonia
Reduction of nitrite and nitrate
Multi-path nitrogen metabolism
