摘要
以山新杨无菌苗幼叶为试材,分别研究了3种选择剂卡那霉素、新霉素、G418和4种押菌剂头孢噻肟钠、头孢唑林钠、头孢曲松钠及羧苄青霉素对山新杨离体叶片不定芽再生的影响。结果表明:浓度为20 mg/L的卡那霉素和浓度为50mg／L的新霉素作为山新杨叶片转化的选择压是合适的,而G418不是山新杨叶片转化的最佳选择剂。在4种押菌抗生素中,头孢唑林钠对不定芽的再生没有影响,但褐化率高,羧苄青霉素抑制不定芽的再生,故二者均不适合作为山新杨遗传转化的抑菌剂;头孢噻肟钠和头孢曲松钠对不定芽的再生影响不大,是山新杨遗传转化的最佳抑菌剂。
An experiment was conducted to study the effects of three selective antibiotics （ Kanamycin, Neomycin, G418） and four bacteriostatie antibiotics （Cefntaxime sodium, Cefazolin sodium, Ceftriaxone sodium, Carbenicillin） on adventitious bud regeneration from in vitro young leaves using Shanxinyang （Populus davidiana Dode x P. boUena Lauche） as testing material. Result showed that Kanamycin and Neomycin are optimal for genetic transformation of Shanxinyang at a dose of 20 mg/L and 50 mg/L, while G418 is not a suitable antibiotic. Among the four kinds of bacteriostatic antibiotics, Cefazolin sodium has no effect on the adventitious bud regeneration but results in a higher browning rate, while Carbenicillin inhibits the adventitious bud regeneration. So both the bacteriostatic antibiotics are not fit for genetic transformation of Shanxinyang. Both Cefotaxime sodium and Ceftriaxone sodium have little effect on the growth of adventitious bud, which arc the best bacteriostatic agents for genetic transformation of Shanxinyang.
出处
《东北林业大学学报》
CAS
CSCD
北大核心
2007年第1期28-30,共3页
Journal of Northeast Forestry University
基金
黑龙江省科技攻关重点项目(GB068303-1)资助。
关键词
山新杨
抗生素
不定芽
Populus davidlana x P. boUena
Antibiotics
Adventitious buds
