摘要
目的：分离鉴定三列凹顶藻Laurencia tristicha中的化学成分，供药理活性筛选。方法：采用正相和反相硅胶柱色谱、凝胶柱色谱、反相高效液相色谱等方法进行分离；应用包括一维和二维NMR等波谱方法鉴定化合物的结构；用MTT法对得到的化合物进行细胞毒活性评价。结果：分离得到5个倍半萜类化合物，分别鉴定为gossonorol（1），7，10-epoxy-arbisabol-11-ol（2），10-epi-7，10-epoxy—arbisabol—11—01（3），3β-hydroxy-5α，6α—epoxy-β-ionone（4），3β-hydroxy-5β，6β-epoxy-β-ionone（5）；对所有化合物在人肿瘤细胞株HCT-8，Bel-7402，BGc-823，A549和HELP,模型上进行了细胞毒活性测定，IC50均大于10．0μg·mL^-1。结论：化合物1—5均为首次从三列凹顶藻中得到，其中化合物1为首次从海洋生物中分离得到；所有化合物对人肿瘤细胞HCT-8，Bel-7402，BGc-823，A549和HELA均无明显活性。
Objective： To isolate and identify chemical constituents of Laurencia tristicha. Method： Compounds were isohted by normal phase silica gel and Sephadex LH-20 gel column chromatography and reverse phase HPLC. Structures were elucidated by spectroscopic methods including 1 D and 2D NMR, IR and MS techniques. Cytotoxicities of the purified compounds were evaluated by MTT method against human cancer cell lines HCT-8, Bel-7402, BGC-823, A.549, and HELA. Result： Five compounds were isolated from L tristicha. Their structures were elucidated as gossonorol （1）, 7, 10-epoxyarbisabol-11-ol （2）, 10-epi-7, 10oepoxyarbisabol- 11-ol （3）, 3β-hydroxy-5α, 6α-epoxy-β-ionone （4）, 3β-hydroxy-5β, 6β-epoxy-β-ionone （5）. Conclusion： Compounds 1-5 were isolated from L. tristicha for the first time. Compound Ⅰ were first reported from the marine organisms. All compounds were inactive against tested cell lines （IC50 〉 10 μg · mL^-1）.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2007年第2期120-123,共4页
China Journal of Chinese Materia Medica
基金
国家自然科学基金(20432030)
新世纪优秀人才支持计划资助("NCET")
