摘要
本文论述了有关人类白血病细胞系K562细胞的体外培养的特点,以及胶体金纳米颗粒独特的物理性质,并应用胶体金治疗肿瘤的原理和意义,成功的在体外条件下培养出生长状态稳定的K562细胞,最后通过观察、记录体外生长的状况,绘制了细胞生长曲线,并计算出细胞的倍增时间。利用柠檬酸三钠来还原氯金酸的方法制备了胶体金,通过紫外／可见分光光度计和电子透射显微镜检测了胶体金的吸收光谱以及它的颗粒直径、形状,进行了胶体金颗粒对K562细胞的影响研究。实验结果表明,加入胶体金和没加胶体金的细胞生长状况基本相同。
In this paper, we first discussed the characteristics about the culture of the K562 cell （a kind of human leukaemia cell line） in vitro and the principle of applying coloidal gold in cancer treatment. Then, we described the experiment of the culture of K562 cell in vitro and the research about the influence of colloidal gold on this kind of call. In the experiment of cell culture, we studied the nutrition and aseptic condition needed by the K562 cell Culture in vitro, constructed the external environment, and colture out the K562 cell which was in good living status. Then we found out the growth and division cycle of the cell by observation, and also made out the cell growth curve by experiment of cell count and live cell detecting. Through the cell growth curve, we figured out the doubling generation time. Colloidal gold is a suspension of gold particles whose diameter range is from 1 to 100 nanometres. We prepared it through liquid reduction with sodium citrate as reducing agent. Transmission electron microscopy （TEM） and UV/visible absorption spectrophotometer were used to check the particle diameter and the shape of colloidal gold. We also investigated the influence of the coloidal gold on K562 cell. We added some of the colloidal gold into the cell culture liquid and observed the cell status. In the experiment, we set a control group which did not add the colloidal gold to compare the result. The result indicated that the colloidal gold had no influence on the living K562 cells.
出处
《现代生物医学进展》
CAS
2006年第12期7-9,共3页
Progress in Modern Biomedicine
基金
国家自然科学基金资助项目(60578026)
