摘要
目的调查铜绿假单胞菌对常用抗生素的耐药特性及氨基糖苷类药物修饰酶基因表达。方法用Phoen ixTM-100系统鉴定细菌和药敏试验。用琼脂扩散法检测20株多重耐药铜绿假单胞菌对阿米卡星、庆大霉素、妥布霉素和奈替米星4种药物的敏感性。采用聚合酶链反应(PCR)扩增4种氨基糖苷类药物修饰酶基因,并使用DNA测序加以证实。结果190株铜绿假单胞菌对氯霉素、四环素、复方磺胺耐药率最高,均为98.9%;对亚胺培南、美洛培南的耐药率分别为15.3%和6.8%。从20株菌中检出aac(6′)-Ⅰ、aac(6′)-Ⅱ和ant(2″)-Ⅰ3种修饰酶基因,阳性率分别为10%、60%和65%,未发现ant(3″)-Ⅰ基因。20株菌对阿米卡星、庆大霉素、妥布霉素和奈替米星的耐药率分别为35.0%、90.0%、70.0%、60.0%。结论本地区铜绿假单胞菌对氨基糖苷类药物的耐药与修饰酶基因的传播表达有关。
Objective To investigate the antimicrobial resistant characteristics and aminoglycoside-modifying enzyme gene expressions in Pseudomonas aeruginosa. Methods Bacteria identification and antimicrobial susceptibility tests were performed by Phoenix^TM-100 system. Antimicrobial susceptibility tests of amikacin, gentamicin, tobramycin and netilmicin in 20 multi-drug resistant Pseudomonas aeruginosa were detected by agar diffusion. Four aminoglycosidemodifying enzyme genes were amplified by pelymerase chain reaction（PCR） and verified by DNA sequencer. Results The resistant rates of Pseudomonas aeruginosa in 190 strains to chloramphenicol, tetracycline and trimethoprim-sulfoxazole were all 98.9%. Imipenem and meropenem showed high antimicrobial activities with resistance of 15.3% and 6. 8%. Three aminoglycoside-modifying enzyme genes including aac（6′）-Ⅰ, aac（6′）-Ⅱ and ant（2″）-Ⅰ were found in 20 strains, and their rates were 10%, 60% and 65%, respectively. But ant（3″）-Ⅰ gene was negative. Twenty isolates resistant to amikacin, gentamicin, tobramycin and netilmicin were 35.0%, 90.0%, 70.0% and 60.0%, respectively. Conclusions Pseudomonas aeruginosa resistant to aminoglycosides in our hospital relates to the spread of aminoglycoside-modifying enzyme genes.
出处
《检验医学》
CAS
北大核心
2007年第1期67-70,共4页
Laboratory Medicine
