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急性白血病LRP15基因启动子区甲基化及其表达分析 被引量:3

LRP15 Gene Promoter Region Methylation and Its Expression in Acute Leukemia
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摘要 为了探讨LRP15基因在急性白血病(AL)患者中的表达情况及其与启动子区CpG岛甲基化的关系,采用甲基化特异PCR(MSP)方法检测AL患者LRP15基因启动子区甲基化状态,并用RT-PCR方法检测该基因在这些患者中的表达情况。结果发现,LRP15基因在缓解与非缓解状态白血病患者中的表达分别为47.6%和16.7%,其启动子区甲基化比例分别为38.1%和72.2%。LRP15基因的表达与其启动子区甲基化之间无相关(P=0.0087)。结论:AL患者LRP15基因启动子区的甲基化不完全是导致LRP15基因表达沉默的原因。 To investigate the relationship between LRP15 gene promoter region methylation and its gene expression in acute leukemia patients, the status of LRP15 gene promoter region methylation was detected by MS-PCR and the gene expression was detected by RT-PCR in bone marrow samples from leukemia patients. The results indicated that the LRP15 gene expression was 47, 6% in complete remission (CR) patients and 16.7% in non-CR patients respectively, while LRP15 gene promoter region methylation was 38.1% in CR group and 72, 2% in non-CR group respectively. No relationship was found between LRP15 gene promoter region methylation and its expression (P = 0. 0087). It is conclu- ded that the methylation in LRP15 gene promoter region may not be the only reason for LRP15 gene silence,
出处 《中国实验血液学杂志》 CAS CSCD 2007年第1期39-41,共3页 Journal of Experimental Hematology
基金 国家自然科学基金(编号39970318) 973国家重点基础研究专项经费资助项目(编号2005CB522400)
关键词 LRP15基因 急性白血病 启动子区甲基化 LRP15 gene acute leukemia promoter region methylation
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参考文献8

  • 1徐周敏,于力,卢学春,韩为东,李续建,靖域,王书红,靳海杰,楼方定.新的白血病复发相关候选基因LRP15全长cDNA的克隆[J].中国实验血液学杂志,2003,11(1):22-26. 被引量:12
  • 2徐周敏,于力,卢学春,韩为东,李续建,靖域,王书红,靳海杰,楼方定.生物信息学研究新基因LRP15[J].生物技术通讯,2002,13(3):187-190. 被引量:10
  • 3徐周敏,于力,卢学春,韩为东,王书红,靖域,赵瑜,靳海杰,楼方定.新基因LRP15的功能预测及其编码蛋白定位[J].军事医学科学院院刊,2003,27(3):183-185. 被引量:5
  • 4Esteller M. CpG island hypermethylafion and tumor suppressor genes: a booming present, a brighter future. Oncogene. 2002;21:5427 - 5440
  • 5Esteller M, Silva JM, Dominguez G, et al. Promoter hypermethylation and BRCA1 inactivation in sporadic breast and ovarian tumors. J Natl Cancer Inst. 2000;92:564 -569
  • 6Baylin SB, Herman JG. DNA hypermethylation in tumorigenesis:epigenefies joins genetics. Trends Genet. 2000 ; 16 : 168 - 174
  • 7Herman JG, Baylin SB. C, ene silencing in cancer in association with promoter hypermethylation. N Engl J Med. 2003 ;349:2042 - 2054
  • 8Issa JP, Zehnbauer BA, Civin CI, et al. The estrogen receptor CpG island is methylated in most hematopoietic neoplasms. Cancer Res,1996 ;56:973 - 977

二级参考文献6

  • 1Weaver RF主编.分子生物学[M].北京:科学出版社,2000.281-282.
  • 2Kusuda J, Hidari N, Hirai M, et al. Sequence analysis of the cDNA for the human casein kinase I delta (CSNKID) gene and its chromosomal localization [J]. Genomics, 1996, 32(1): 140- 143.
  • 3Feliciello A, Gallo A, Mele E, et al. The localization and activity of cAMP-dependent protein kinase affect cell cycle progression in thyroid cells[J]. J Biol Chem,2000,275(1):303-311.
  • 4J.萨姆布鲁克 E.F弗里奇 T.曼尼阿蒂斯(金冬雁等译).分子克隆实验指南:第2版[M].北京:科学出版社,1992.880-897.
  • 5于力,韩为东,楼方定,王全顺,赵瑜,MichaelACaligiuri.新的白血病相关基因LRP16的克隆[J].军医进修学院学报,2000,21(2):81-84. 被引量:59
  • 6徐周敏,于力,卢学春,韩为东,李续建,靖域,王书红,靳海杰,楼方定.新的白血病复发相关候选基因LRP15全长cDNA的克隆[J].中国实验血液学杂志,2003,11(1):22-26. 被引量:12

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