摘要
目的:探讨膀胱移行细胞癌中MDR1基因(P-糖蛋白)与Fas、Survivin表达的相互关系及其与膀胱癌生物学行为的相关性。方法:应用免疫组织化学方法检测64例膀胱移行细胞癌和12例正常膀胱粘膜中P-GP、Fas、Survivin的表达。结果:膀胱移行细胞癌中P-GP、Survivin的表达阳性率高于正常粘膜,与膀胱癌的分级有关(P<0.01);而Fas在膀胱正常粘膜中表达高于移行细胞癌,差异有统计学意义(P<0.01)。复发性膀胱癌P-GP的阳性表达率高于初发膀胱癌(P<0.01)。膀胱移行细胞癌P-GP的表达与Fas呈负相关,而与Survivin表达无关。结论:膀胱移行细胞癌的MDR1(多药耐药基因)与Fas的表达密切相关,而与凋亡抑制蛋白Survivin的表达无关。本研究为采用MDR逆转剂或Fas干扰剂以增加膀胱移行细胞癌的化疗敏感性提供实验依据。
Objective： To explore the interrelation between expression of MDR1/P-glycoprotein, Fas and Survivin and to study the correlation between these expression levels and the biological behavior of transitional cell carcinoma of the bladder （TCCB）. Methods： Immunohistochemistry was used to detect the expression of P-GP, Survivin and Fas in TCCB （n=64） and normal bladder mucosa （n=12）. Results： The expression level of P-GP and Survivin in TCCB was higher than those levels found in normal bladder mucosa （P〈0.01）. The expression levels of P-GP and Survivin were strongly correlated with clinical stage （P〈0.01）. Fas was detected in 50% of the TCCB cases and in 100% of the normal bladder mucosa samples （P〈0.01）. P-GP expression in recurrent TCCB was higher than in primary TCCB （P〈0.01）. The expression of P-GP in TCCB had a negative correlation with that of Fas, but had no correlation with Survivin expression. Conclusions： Multiple drug resistance in TCCB is strongly correlated with the expression of P-GP and Fas, but not with Survivin expression. Our experimental evidence suggests that chemosensitivity may be increased by using MDR reversing agents or Fas interfering agents.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
2007年第2期82-85,共4页
Chinese Journal of Clinical Oncology
基金
天津市高等校科技发展基金资助(编号:20030304)
