期刊文献+

PrP及其缺失突变体与微管蛋白的体外相互作用的初步研究 被引量:1

Interaction Analysis between Various PrP Fusion Proteins and the Tubulin In Vitro
下载PDF
导出
摘要 为了进一步确定PrP蛋白与微管蛋白是否发生分子间相互作用以及PrP蛋白多肽链中与微管蛋白相互作用的区域,我们表达纯化了全长的PrP以及PrP蛋白缺失突变体,提取了兔脑组织中天然微管蛋白。利用pull-down及免疫共沉淀方法检测全长PrP及PrP蛋白缺失突变体与微管蛋白是否发生分子间相互作用。结果显示,全长His-PrP23-231能与微管蛋白发生体外相互作用,并首次证实了PrP与微管蛋白相互作用的区域位于PrP N端第23位至91位氨基酸。此研究为进一步研究PrP在神经细胞的主动转运机制以及Prion疾病的发病机制提供了一定的理论基础。 In order to further study the potential interaction between PrP protein and the tubulin and identify the binding region in PrP with tubulin, native tubulin was extracted from rabbit brian tissues, while various recombinant PrP proteins were expressed and purified. The possible molecular interaction between various PrP fusion proteins and tubulin was tested by pull-down and immunoprecipitation assays. Remarkable molecular interaction between the full length PrP and tubulin was observed by pull-down and immunoprecipitation assays. Subsequently, the binding regions within PrP with tubulin were firstly mapped in the aa 23 - 91 region within N-terminus of PrP peptide. The studies of the association of PrP with tubulin may further provide insight into the unresolved mechanism of active transport of PrP protein in neurons and possible cellular pathways by which prion causes disease.
出处 《病毒学报》 CAS CSCD 北大核心 2007年第1期28-32,共5页 Chinese Journal of Virology
基金 国家自然科学基金委重点项目(编号:3011402) 国家自然科学基金委重点项目(编号:30130070) 国家自然科学基金委项目(编号:30571672) 国家高技术研究发展计划(863计划)项目(编号:2001AA215391) 欧盟项目(编号:QLRT200001441) 国家科技攻关计划项目(编号:2003BA712A04-02)资助
关键词 PrP蛋白 微管蛋白 分子间相互作用 prion protein tubulin molecular interaction
  • 相关文献

参考文献17

  • 1Prusiner S B.Prions[J].Proc Natl Acad Sci USA,1998,95:13363-13383.
  • 2Legname G,Baskakov I V,Nguyen H O,et al.Synthetic mammalian prions[J].Science,2004,305:673-676.
  • 3Hornshaw M P,McDermott J R,Candy J M.Copper binding to the N-terminal tandem repeat regions of mammalian and avian prion protein[J].Biochem Biophys Res Commun,1995,207:621-629.
  • 4Stockel J,Safar J,Wallace A C,et al.Prion protein selectively binds copper(Ⅱ) ions[J].Biochemistry,1998,37:7185-7193.
  • 5Brown D R,Clive C,Haswell S J.Antioxidant activity related to copper binding of native prion protein[J].J Neurochem,2001,76:69-76.
  • 6Brown D R,Wong B S,Hafiz F,et al.Normal prion protein has an activity like that of superoxide dismutase[J].Biochem J,1999,344:1-5.
  • 7Hachiya N S,Watanabe K,Sakasegawa Y,et al.Microtubules-associated intracellular localization of the NH2-terminal cellular prion protein fragment[J].Biochem Biophys Res Commun,2004,313:818-823.
  • 8Borchelt D R,Koliatsos V E,Guarnieri M,et al.Rapid anterograde axonal transport of the cellular prion glycoprotein in the peripheral and central nervous systems[J].J Biol Chem,1994,269:14711-14714.
  • 9韩俊,王小凡,姚海兰,高晨,李锋,张宝云,姜慧英,董小平.PrP可抑制tau介导的体外微管形成作用[J].神经科学通报,2005,21(6):398-403. 被引量:3
  • 10Maccioni R B,Cambiazo V.Role of microtubule-associated proteins in the control of microtubule assembly[J].Physiol Rev,1995,75:835-864.

二级参考文献32

  • 1姚海兰,聂凯,韩俊,肖新莉,陈岚,王小凡,周伟,姜慧英,蔡昌学,董小平.一种新型原核表达载体的构建及应用[J].华中科技大学学报(医学版),2004,33(5):522-525. 被引量:6
  • 2Aguzzi A, Montrasio F, Kaeser PS. Prions: health scare and biological challenge. Nat Rev Mol Cell Biol, 2001,2:118-126.
  • 3Prusiner SB. Pfions, Proe Natl Aead Sei USA, 1998, 95:13363-13383.
  • 4Borchelt DR, Koliatsos VE, Guamieri M, et al. Rapid anterograde axonal transport of the cellular priori glycoprotein in the peripheral and central nervous systems. J Biol Chem, 1994, 269: 14711-14714.
  • 5Ferrer I. Synaptie pathology and cell death in the cerebellum in Creutzfeldt-Jakob disease. Cerebellum, 2002, 1 (3): 213-222.
  • 6Ishizawa K, Komofi T, Shimazu T, et al. Hyperphosphorylated tau deposition parallels pfion protein burden in a case of Gerstmann6-Straussler-Scheinker syndrome P102L mutation complicated with dementia. Acta Neuropathol (Berl) , 2002, 104:342-350.
  • 7Spittaels K, Van den Haute C, Van Dorpe J, et al. Prominent axonopathy in the brain and spinal cord of transgenic mice overexpressing four-repeat human tan protein. Am J Pathol, 1999, 155(6):2153-2165.
  • 8Sloboda RD, Belfi LM. Purification of tubulin and mierotubule-associated proteins by membrane ion-exchange chromatography. Protein Expr Purlf, 1998, 13(2) : 205-209.
  • 9Hegde RS, Tremblay P, Groth D, et al. Transmissible aud genetic prion diseases share a common pathway of neurodegeneration [J].Nature, 1999, 402:822-826.
  • 10Cohen E, Taraboulos, A. Scrapie-like pfion protein accumulates in aggresomes of cyclospofin A-treated cells. EMBO J, 2003, 22:404-417.

共引文献5

同被引文献3

引证文献1

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部