摘要
以IFN-γ为靶点,探讨它与SLE疾病活动性和肾脏损害的关系,从而探讨SLE的发病机制。32例SLE患者,根据活动指数评分,将病人分为活动期和稳定期两组。同时,按24 h尿蛋白定量等将SLE患者分为狼疮肾炎(lupus nephritis,LN)组和非肾炎组。另设健康志愿者为对照组。采用三色流式细胞术检测32例SLE患者外周血IFN-γ水平。流式细胞仪检测细胞培养后CD45+IFN-γ+的百分率情况;IFN-γ表达百分率在不同分组中表达:SLE活动组>SLE稳定组>对照组。在合并肾脏损害患者中,总淋巴细胞表达IFN-γ的阳性百分率:LN组>非LN组。IFN-γ水平与SLEDAI评分呈正相关(P<0.01)。表明IFN-γ水平增高与SLE疾病活动性呈正相关,建议可作为判断SLE疾病活动临床参考指标之一;抗Blys单抗下调IFN-γ的表达可作为检测该单抗干扰SLE免疫功能的靶点之一。
By using IFN-γ as the target of study, its relationship to the disease activity and renal damages of SLE was investigated in order to clarify the pathogenesis of this illness according to the evaluation with activity index, 32 SLE patients were divided into active and stable groups, and based on the quantitative determination of urine albumin at 24 hours, they were divided into lupus nephritis （LN） and non-LN groups. In addition, 20 healthy volunteers were closed as the control group. Three colored flow cytometry was used to detect the level of IFN-γ in the peripheral blood. As demonstrated by flow cytometry analysis, the percentages of CD45^＋ IFN-γ^＋ cells in the cell cultures and the expression of IFN-γ in patients with active SLE were higher than those in patients with stable SLE and healthy controls. In patients complicated with renal damages, the positive percentages of the IFN-γ level in the LN-groups were higher than those in the non-LN group of patients. A significant positive correlation between the SLEDAI scores and the percentage of IFN-γ expression could be demonstrated. These results indicate that positive correlation exists between the higher level of IFN-γ expression and the activity of disease and this may be used as a reference index for the acitivities of SLE.
出处
《现代免疫学》
CAS
CSCD
北大核心
2007年第1期69-72,共4页
Current Immunology
基金
深圳市医学重点学科建设经费资助项目(深卫科发[2005]25号)
关键词
系统性红斑狼疮
发病机制
IFN-Γ
流式细胞术
systemic lupus erythematosus
pathogenesis
interferon-γ
γδT cell
flow cytometry
