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鸡胚胎干细胞的分离、培养与鉴定的初步研究 被引量:5

Pilot study of isolation,culture and identification of stem cells in chicken embryo
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摘要 采用饲养层培养法,以高糖DM EM为基础培养基,同时添加胎牛血清以及5 ng/m l SCF,l0 ng/m l bFGF和1 000 IU/m l mL IF等细胞因子与鸡胚浸出液等,对鸡的X期胚盘细胞进行离体培养,并利用形态学鉴定、AKP染色、体外分化实验等方法对所获得的细胞克隆进行鉴定。实验结果表明,本实验建立的培养体系培养出了具有多分化潜能的类胚胎干细胞。 The feeder layer was used to culture the embryonic stem cells. The X-stage blastoderm cells were cultured in vitro with DMEM (high glucose) as base culture medium supplemented with fetal calf serum, 5 ng/ml SCF, 10 ng/ml bFG, 1 000 IU/ml mLIF. The cell clone was identified by morphologic method, AKP dying and differentiation experimentation in vitro . The results indicated that the pluripotency stem cells can be obtained in a suitable culture system built in this study.
出处 《佛山科学技术学院学报(自然科学版)》 CAS 2006年第4期72-75,共4页 Journal of Foshan University(Natural Science Edition)
基金 广东省自然科学基金资助项目(5012371)
关键词 胚胎干细胞 培养 chicken embryonic stem cells culture
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  • 1安静,杜立新.鸡胚胎干细胞的分离、培养和鉴定[J].动物学报,2003,49(5):698-703. 被引量:21
  • 2EVANS MJ,KAUFMAN MH.Establishment in culture of pluripotential cells from mouse embryos[J].Nature,1981,292(5819):154-156.
  • 3ETCHES RJ,CARASIENCE RS,CLARK ME.Chimeric chickens and their use in manipulation of the chicken's genome[J].Poultry Science,1993,72(5):882-889.
  • 4PETITTE JN,CLARK ME,GLIU,et al.The production of somatic and germline chimeras in the chick by transfer of early blastodermal cell[J].Development,1990,108(1):185-190.
  • 5NATIO M,WATANABE M,KINUTANI M.Production of quail-chick chimaeras by blastodermal cell trabsfer[J].Br.Poult,1991,32(1):79-86.
  • 6PAIN B,CLARK M E,SHEN M,et al.Long-term in vitro culture and characterization of avian embryonic stem cells with multiple morphogenetic potentialities[J].Development,1996,122(8):2339-2348.
  • 7KINO K,PAIN B,LEIBO SP.Production of chicken chimeras from injection of frozen-thawed blastodermal cells[J].Poultry Science,1997,76(5):753-760.
  • 8杜立新,李善刚.鸡胚胎体外培养和嵌合体制作的研究[C].第十次全国动物遗传育种学术讨论会论文集.1999:467-472.
  • 9刘民,李柏青.小鼠胚胎成纤维细胞的分离、扩增和抑制[J].中国实验动物学杂志,2002,12(4):215-219. 被引量:30
  • 10杨景山.碱性磷酸酶的显示方法和原理.医学细胞化学与细胞生物技术[M].北京:北京医科大学和中国协和医科大学联合出版社,1989:41-44.

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