摘要
采用高效液相色谱法同时测定鸡蛋中的VA和VE含量,样品经皂化后,用石油醚—乙醚萃取,浓缩挥干,用异丙醇溶解后进样。以C18为固定相,甲醇—水(93∶7)为流动相,流速1.0mL/min,采用双波长检测,分别为325nm处测定VA,290nm处测定VE。结果VA含量为0.00151～0.08300g/L,与色谱峰面积呈良好的线性关系;VE含量为0.00531～0.29200g/L,与峰面积呈线性关系。采用HPLC法使鸡蛋中的VA和VE含量测定快速,灵敏,简便易行。
A method for the simultaneous determination of vitamin A and E in egg yolk by high performance liquid chromatography was developed. The sample was determined after being saponified, abstracted with petroleum ether and solved by isopropanol. The C18 column （5μm, 250mm ×4.6mm） was selected and the mobile phase consisted of carbinol-water （93 ： 7） at a flow rate of 1.0mL/min. The detection wavelength was 325nm for vitamin A and 290nm for vitamin E. The linear range of vitamin A was between the concentration of 0.001 51 and 0.083g/L; the linear range of vitamin E was between the concentration of 0.005 31 and 0.292g/L. This method is rapid and sensitive, accurate and feasible.
出处
《农产品加工（下）》
2007年第2期58-61,64,共5页
Farm Products Processing
