摘要
为考察环境条件对紫球藻B-藻红蛋白(B-PE)合成的影响,分别在不同的光质、光强和无机氮盐条件下培养紫球藻,并利用硫酸铵沉淀法和羟基磷灰石(HA)柱层析等方法对B-PE进行了分离提取.结果表明,HA柱层析法能够有效地从紫球藻细胞水溶性色素粗提物中分离得到高纯度的B-PE,经检测其纯度(OD545/OD280)达到了4.32.培养条件对B-PE在细胞内的积累有一定的影响,绿光能显著提高紫球藻粗蛋白及B-PE含量,两者分别占细胞干重的24.56%和9.14%,高于自然光下的17.78%和5.3%;高光照有利于B-PE的积累,并且随着光照强度的增加,藻体细胞干重、粗蛋白及B-PE的含量均随之增加.同等光照强度下,氮盐含量对B-藻红蛋白的积累有较显著的影响,当氮盐浓度为3.2g·L-1时,紫球藻细胞的生长速度较快,B-PE的含量也较高,占粗蛋白总量的88.43%.
The variations in culture conditions determine different content of B-phycoerythrin（B-PE） from Porphyridium cruentum. In order to investigate the effect of culture conditions on B-PE synthesis, the cells of P. cruentum were cultured by different cultural parameters such as light quality, irradiance intensity, and nitrogen concentration, and then the B-PE was separated from soluble pigments of P. cruentum using the methods of deposited in 55 % （W/V）solid ammonium sulfate and chromatographer on a hydroxylapatite column（HA）. The results showed that the HA-column chromatographic method could purify the B-PE efficiently, and the purity of B-PE reached to 4.32 （OD545/OD280）. The environmental factors could remarkably affect the B-PE contents in the P. cruentum cells. The green light could enhance the content of crude protein and B-PE compared with white light, and under appropriate conditions, both 24.56% crude protein and 9.14% B-PE were obtained respectively. In a defined range, the high light intensity was propitious to accumulation of B-PE in the cells, and with the increasing of light density, the dry weight of cell biomass and the content of proteins were also improved. At the same light intensity, the impact of nitrogen concentration on the yield of B-PE in the cells was notable. When the concentration of nitrogen was 3.20 g· L^-1, both the growth rate of the cells and the content of B-PE arrive to their maximum value, and the content of B-PE accounted for 88.43% of total crude proteins in the cells.
出处
《浙江大学学报（农业与生命科学版）》
CAS
CSCD
北大核心
2007年第1期40-44,共5页
Journal of Zhejiang University:Agriculture and Life Sciences
基金
教育部留学回国人员科研启动基金(HY03L1).
关键词
紫球藻
B-藻红蛋白
分离纯化
培养条件
Porphyridium cruentum
B-phycoerythrins
separation and purification
culture conditions
