摘要
通过单因素浓度试验和正交试验研究了5种金属离子、不同激素组合及不同天然附加物对铁皮石斛类原球茎增殖、分化的影响。试验结果表明,5种金属离子对类原球茎增殖影响的主次顺序为Mg2+(Fe2+(Ca2+(Mn2+(Zn2+,类原球茎增殖的最佳培养基为1/2MS+NAA 0.5mg/L+10%马铃薯汁+3%蔗糖(含Mg2+1.0 mmol/L、Ca2+2.0 mmol/L、Fe2+0.1mmol/L、Mn2+0.1mmol/L和Zn2+0.03 mmol/L)。培养基1/2MS+NAA 0.5mg/L+20%马铃薯汁+蔗糖20g/L+琼脂0.8g/L最适于铁皮石斛类原球茎的分化。
This research is concemed with redifferentiation optimization of five metal ions, different hormone and natural juice for protocorm - like body（PLBs） proliferation in liquid culture of Dendrobium candidum by one factor at a time and orthogonal test. Results showed that, the effects of metal ions on PLBs proliferation were significant and the order was Mg^2＋〉 Fe^2＋ 〉 Ca^2＋ 〉 Mn^2＋ 〉 Zn^2＋ . The optimal medium for PLBs proliferation was determined as 1/2 MS ＋ NAA0.5mg/L ＋ 10% PE ＋ 3% sugar（ containing Mg^2＋ 1.0 mmol/L, Ca^2＋ 2.0 mmol/L, Fe^2＋ 0.1 mmol/L, Mn2 ＋ 0. 1mmol/L and Zn^2＋ 0. 03 mmol/L）. The optimal medium for PLBs redifferentiation was determined as 1/2MS ＋ NAA0. 5mg/L ＋20% PE ＋ sugar 20g/L ＋ agar 0.8g/L.
出处
《江西农业学报》
CAS
2007年第1期84-86,91,共4页
Acta Agriculturae Jiangxi
基金
宁德市科技计划项目(编号为2004Z12)
关键词
铁皮石斛
类原球茎
金属离子
增殖
分化
Dendrobium candidum
Protocorm-like bodies （PLBs）
Metal ion
Proliferation
Redifferentiation
