摘要
目的探讨两亲性生物降解材料聚乳酸-聚乙二醇(MPEG-PLA)键合紫杉醇(paclitaxel)纳米微粒对Lewis肺癌细胞株的增殖抑制作用。方法应用透析方法制备化学键合的紫杉醇纳米粒子,并用动态激光光散射(DLS)测定其粒径和扫描电镜观察其形态,通过MTT法测定和比较键合紫杉醇纳米微粒与紫杉醇对Lewis肺癌细胞株的增殖抑制作用。结果键合紫杉醇纳米粒子的粒径分布基本呈现单一对称分布,能够形成球状,其和紫杉醇相比较,对Lewis肺癌细胞株的增殖具有抑制作用,并且具有浓度和时间依赖性,随药物作用时间和浓度的增大,抑制率逐渐增高。结论两亲性生物可降解材料聚乳酸-聚乙二醇(MPEG-PLA)键合紫杉醇(paclitaxel)纳米微粒,与紫杉醇同样具有抗癌活性,可做为肺癌靶向治疗的新型制剂。
Objective To study the inhibitory effect of PEG - PLA - paeiitaxel conjugate micelles nanometer particle against lewis lung cancer. Methods PEG - PLA - paclitaxel conjugate micelles nanometer particle was generated by dialysis and its physicochemical characteristicss were evaluated by dynamic laser light scattering （DLS） and scanning electron microscope （SEM）. Antitumor activities against lewis lung cancer cells were evaluated by MTT method. Results The nanometer particle assumed a spherical shape and had a unimodal size distribution. It had a sustained antitumor activity against lewis lung cancer cells in a time - and dose - dependent manner. Conclusion PEG - PLA - paclitaxel conjugate nanometer particle had a sustained antitumor activity against lewis lung cancer cells similarto paclitaxel. It can be used as a new fargeted therapy for lung cancer.
出处
《广东医学》
CAS
CSCD
北大核心
2007年第2期175-177,共3页
Guangdong Medical Journal
基金
国家自然科学基金资助项目(编号:20274048)
关键词
紫杉醇
肺癌
靶向治疗
Paclitaxel Lung cancer Targeted therapy
