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谷氨酸作用下神经元凋亡诱导因子核转位 被引量：1

Translocation of apoptosis-inducing factor from mitochondria to nuclei of cortical neurons following glutamic acid treatment
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摘要 目的:研究体外培养的皮质神经元在谷氨酸作用下,其凋亡诱导因子(AIF)向细胞核转位的情况。方法:用谷氨酸诱导体外培养的皮质神经元凋亡,用免疫荧光指示热休克蛋白-60(HSP-60)和AIF的亚细胞分布,4,6-二脒-2苯-基吲哚(DAPI)显示细胞核,并根据DAPI显示的细胞核形态及其位置判断细胞凋亡。结果:所有神经元都表达AIF。正常情况下AIF分布在线粒体;经谷氨酸处理后AIF从线粒体释放,并可转位至细胞核,致部分细胞可能发生凋亡。结论:谷氨酸可引起AIF从线粒体释放并向细胞核转位,同时可诱导体外培养的原代皮质神经元凋亡。 Objective：To investigate the apoptosis-inducing effects of glutamic acid on primary cultures of rat cortical neurons, and on the translocation of apoptosis-inducing factor （AIF） from mitochondriato nuclei. Methods：The apoptosis of cortical neurons was induced by glutamic acid. The subcellular distribution of AIF and heat shock protein 60 （HSP-60） indicating the location of cytosol was revealed by immunofluorescence, with 4,6-diamidino-2-phenylindole （DAPI） indicating nuclei. Cellular apoptosis was judged by the nuclear modality and the position relative to cytoplasm showed by DAPI. Results：Almost all neurons expressed AIF. AIF located in mitochondria for the normal neurons and in some extent translocated from mitochondria to nuclei in part of the neurons, after treated with glutamic acid. Conclusion：Glutamic acid could bring about the translocation of AIF from mitochondria to nuclei and induce cortical neurons to undergo apoptosis.
作者 许康 柯贤军 郭珍立 邓晓红 梁燕玲
机构地区 湖北省新华医院脑科中心神经内科 华中科技大学同济医学院附属同济医院神经内科
出处 《医学研究生学报》 CAS 2007年第2期132-134,I0003,共4页 Journal of Medical Postgraduates
基金 国家自然科学基金面上项目基金资助(批准号:30070825)
关键词 凋亡诱导因子 谷氨酸 皮质神经元 Apoptosis-inducing factor Glutamic Acid Cortical neurons
分类号 Q244 [生物学—细胞生物学]
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参考文献9

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同被引文献21

  • 1刘小红,赵永利,徐曼,王燕,李明,周熙惠,李正浩.高压氧对缺氧缺血性脑损伤新生大鼠神经细胞凋亡以及凋亡诱导因子核转移的抑制作用[J].中国儿童保健杂志,2006,14(6):599-602. 被引量：7
  • 2华正宇,赵瑞波,刘宏.亚低温对脑缺血性损伤后凋亡诱导因子表达变化的影响[J].哈尔滨医科大学学报,2007,41(2):147-150. 被引量：2
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  • 5Lorenzo HK, Susin SA, Penninger J, et al. Apoptosis inducing factor (AIF):a phylogenetically old, caspase independent effector of cell death. Cell Death Differ, 1999,6(6) : 516-524
  • 6Mate MJ,Ortiz- Lombardia M, Boitel B, et al. The crystal structure of the mouse apoptosis inducing factor AIF. Nat Struct Biol, 2002,9 (6) :442-446
  • 7Ishitsuka K, Hideshima T, Hamasaki M, et al. Novel inosine monophosphate dehydrogenase inhibitor VX- 944 induces apoptosis in multiple myeloma cells primarily via caspase- independent AIF/Endo G pathway. Oncogene, 2005,24(38) : 5888 - 5896
  • 8David JP, Wei Wang, Michael B. et al. The Raf Inhibitor BAY 43 - 9006 (Sorafenib) Induces Caspase- Independent Apoptosis in Melanoma Cells. Cancer Res. , 2006,66:1611 - 1619
  • 9Tanaka S, Takehashi M, lida S, et al. Mitochondrial impairment induced by poly(ADP- ribose) polymerase- 1 activation in cortical neutons after oxygen and glucose deprivation. J Neutochem, 2005,95 ( 1 ) : 179-190
  • 10Hong Y, Celine C, Nicolas CS, et al. DNA binding as a structural requirement for the apoptogenic action of AIF. Nat. Struct. Bioi, 2002, 9,680 - 684

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医学研究生学报
2007年 第2期

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