摘要
目的建立适合大规模纯化质粒DNA的工艺。方法采用碱裂解、中空纤维超滤浓缩、疏水层析、分子筛等分离技术纯化质粒DNA。结果纯化后的质粒DNA纯度达95%以上,整个工艺流程总产率为58%,各项指标均符合药用质粒DNA质量标准。结论此纯化工艺简便,产率较高,为DNA疫苗大规模纯化奠定了基础。
Objective To develop a procedure for large-scale purification of plasmid DNA. Methods Purify plasmid DNA by a procedure consisting of alkaline lysis, ultrafltration with hollow fiber filter,hydrophobie chromatography and molecular sieve chromatography. Results The purified plasmid DNA reached a purity of more than 95% and met the requirements for plasmid DNA for medical use. The yield of the procedure was 58%. Conclusion The developed purification procedure was simple and of high yield. It laid a foundation of large-scale purification of DNA vaccine.
出处
《中国生物制品学杂志》
CAS
CSCD
2007年第2期133-135,共3页
Chinese Journal of Biologicals
关键词
质粒
超滤
疏水层析
Plasmid DNA
Ultrafiltration
Hydrophobic chromatography
