摘要
本文研究了单键DNA分子（ssDNA）在石墨电极上的固定方法，采用核酸分子杂交技术，使具有电化学活性的米托蒽醌（MX）嵌入DNA分子双螺旋结构的碱基对中，在电极上形成dsDNA－MX层，通过伏安法研究DNA分子和MX相互作用的电化学行为，并且根据嵌入剂MX的氧化峰电流计算出DNA分子与MX的表观缔合常数K＝8．4×104（mol／L）－1，结合位点的大小n＝5。
A synthesized 75-mer single -stranded DNA- 1 (ssDNA-1 ) was covalently immobi lizccl onto an aminated graphite electrode surtace . At 42℃' in 2 × SSC hybridization buffer solution containing 1. 0mg/L complementary ssDNA-2 and 1. 0 ×10- 3 mol /L mitoxanthrone (MX ). the ssDNA-1 immobilized onto the electrode hybriclizes with ssDNA-2 forming dou ble stranded DNA (dsDNA ). MX was capable of intercalating between base pairs of the dsl)NA layer on the electrode . The electrochemical behaviour of the dsDNA and MX intercala tion was studied by electrochemical signals of MX. The apparent association constant of MX-DNA is 8. 4 × 104 (mol/L ) - 1, and the binding size is 5.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
1996年第11期1301-1304,共4页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金
关键词
核酸分子杂交
嵌入作用
伏安法
DNA
MX
电化学
Deoxyribonucleic acid, mitoxanthrone, hybridization, intercalation,voltammetry
