摘要
目的探讨慢性乙型肝炎患者外周血单个核细胞(PBMC)中部分CXC趋化因子(IP-10、Mig)mRNA表达水平,并比较2种类型干扰素-α(IFN-α)联合利巴韦林治疗后对其表达水平的影响。方法对2004年1月至2005年6月淮南第二矿工医院感染科收治的45例慢性乙型肝炎患者以实时聚合酶链反应(Real-timePCR)法动态检测其经IFN-α联合利巴韦林治疗前、治疗3个月、6个月后PBMC内IP-10、MigmRNA的含量,同时选取同期24例体检正常献血员为对照组,测量其PBMC内IP-10、MigmRNA的含量。2组均以趋化因子与管家基因GAPDH的对数比值为其最终相对表达量。结果慢性乙型肝炎患者治疗前IP-10、MigmRNA表达水平分别为(0.7387±0.0768)、(0.6883±0.0693),显著高于对照组(P<0.001)。IFN-α治疗3个月后IP-10、MigmRNA表达水平分别为(0.5741±0.0912)、(0.5962±0.0987),与治疗前相比,差异有显著性(P<0.05～P<0.001)。IFN-α治疗6个月后IP-10、MigmRNA表达水平分别为(0.4235±0.0924)、(0.4105±0.0898),与治疗前相比,差异有显著性(P<0.001)。普通IFN-α和聚乙二醇化干扰素-α(PEG-IFN-α)治疗后各趋化因子表达水平不同,差异有显著性(P<0.05～P<0.001)。结论慢性乙型肝炎患者PBMC中IP-10、MigmRNA表达升高,普通IFN-α和PEG-IFN-α均能显著下调IP-10、MigmRNA表达水平,PEG-IFN-α治疗组疗效优于普通IFN-α组。IFN这种免疫调节作用,对降低患者体内炎性反应、控制病情发展十分有利。
Objective To study the levels of some CXC chemokines mRNA in patients with chronic hepatitis B and its changes after combinative treatment of two types interferon-α （IFN-α） with Ribavirin. The logarithm rate of chemokine/ GAPDH was regarded as the extreme level of chemokine, Methods The different levels of IP-10 and Mig mRNA in peripheral blood mononuclear cells （PBMC）of chronic hepatitis B were kinetically detected by real-time polymerase chain reaction（real-time PCR）in different treatment stages, Results The expression levels of IP-10 and Mig mRNA in peripheral blood of patients were（0.7387 ± 0.0768 ）, （0. 6883 ± 0.0693 ）, respectively, Which were significantly higher than that in normal controls（ P 〈 0, 001 ）, After treatment for three months ,the expressive levels of IP-10 and Mig mRNA were （0. 5741 ± 0. 0912）, （0. 5962 ± 0, 0987 ）, respectively. There was significant difference between before treatment ＂and after treatment（ P 〈 0. 05 - P 〈 0. 001 ）. After treatment for six months, the expressive levels of IP-10 and Mig mRNA were （0.4235±0.0924）, （0.4105 ± 0. 0898 ）, respectively, There was higher significant difference between before treatment and after treatment（P 〈 0. 001 ）, There was difference for the expression of ehemokines between the general IFN-α and PEG-IFN-α （P 〈 0. 05- P 〈 0, 001 ）. Conclusion This study shows that the expression of IP-10 and Mig mRNA are high in patients with chronic hepatitis B, Both the general IFN-α and PEG-IFN-α can conspicuously down-regulate the levels of IP-10 and Mig mRNA to adjacent normal level in patients with chronic hepatitis B. The curative effect of PEG-IFN-α group is better than that of IFN-α group. IFN-α plays an important role in down-regulating inflammation response, controlling the development of the patients＇ condition.
出处
《中国实用内科杂志》
CAS
CSCD
北大核心
2007年第4期285-288,共4页
Chinese Journal of Practical Internal Medicine
基金
安徽省教育厅自然科学基金(2003kj027zd)
