摘要
实验用兔单特异抗艰难梭菌Ａ毒素ＩｇＧ包被酶标板，以羊抗艰难梭菌Ａ毒素ＩｇＧ标记辣根过氧化物酶作为第二抗体，采用双抗体夹心ＥＬＩＳＡ法检测艰难梭菌Ａ毒素，可检测出０．９４ｎｇ的精制Ａ毒素，对６１株菌的培养液及６５份健康人粪便标本检测发现此法具有较高的特异性。用平行线定量法对几份典型产毒培养物进行了定量测定，结果表明，在一定剂量范围内线性及平行性好，结果准确、可靠。可用于临床粪便标本中艰难梭菌Ａ毒素的筛查及定量检测。
In this paper a indirect enzyme linked immunosorbent assay(ELISA)for detection and quantitation of Clostridium difficile toxin A was developed by using of rabbit mono specific antibody.Using this method,about 0.94ng of purified toxin A could be detected.This method had been used to detect the toxin A in the culture supernatants of 61 tested strains and 65 fecal specimens from healthy persons,which was not reactive with other species of clostridia and toxigenic entero bacteria,and which was in accordance with the bioassays in all of tested strains.In addition,the results also shown that the method was exact and reliable by using this ELISA in combination with the parallel line assay to quantitate the toxin A.These results indicated that the ELISA is a sensitive,specific and suitable method for detection of Clostridium difficile toxin A.
出处
《微生物学免疫学进展》
1996年第4期7-11,共5页
Progress In Microbiology and Immunology
基金
卫生部科研基金
