摘要
目的:观察复方制剂祛毒增宁胶囊(ZL-1)药物有效组分人衔草(JH)与HBsAg蛋白的结合情况,及其体内外抗乙型肝炎病毒的作用．方法:HepG2．2．15与不同浓度JH共培养,采用ELISA法HBsAg和HBeAg的分泌情况．建立鸭乙型肝炎病毒感染模型,以不同剂量JH进行治疗,观察鸭血清乙型肝炎病毒DNA(DHBV- DNA)的变化．结果:JH作用于HepG2．2．15细胞8 d后,对细胞的半数毒性浓度(TC_(50))为1126．01 mg/L,对HBsAg和HBeAg分泌量的的半数抑制浓度(IC_(50))分别为17．52 mg/L和754．26 mg/L,对HBsAg和HBeAg的治疗指数(TC_(50)/IC_(50))分别为64．27和1．49．JH 0．8 g/kg能降低感染鸭血清DHBV-DNA水平,给药后5、10 d和停药后3 d DHBV-DNA吸光度(A)值分别为0．660±0．07, 0．632±0．03,0．663±0．05,与盐水组0．872±0．08比较,有显著性差异(P＜0．05)．结论:JH有抑制乙肝病毒的作用．
AIM： To study the anti-viral effect of Ren Xian Cao （RXC）, extracted from compound agent Qiedu Zengning capsule ZL-1, on the replication of hepatitis B virus （HBV）.
METHODS： HepG2.2.15 cells were co-cultured with different concentrations of RXC, and enzyme-linked immunosorbent assay （ELISA） was used to examine the secretion of HBsAg and HBsAg. Duck models of HBV infection was established by intravenous injection of duck HBV, and then treated with different doses of RXC. The changes of duck HBV DNA were observed.
RESULTS： After RXC addition for 8 days, the half toxic concentration （TC50） of RXC was 1126.01 mg/L, and the half inhibitory concentration （IC50） on the secretion of HBsAg and HBeAg was 17.52 and 754.26 mg/L, respectively. The value of therapeutic index （TC50/IC50） for HBsAg and HBeAg was 64.27 and 1.49, respectively. RXC at a dose of 0.8 g/kg significantly lowered the level of serum duck HBV DNA at the 5^th, 10^th day of treatment and 3 days after the end of treatment as compared with normal saline did （absorbency：0.660 ± 0.07, 0.632 ±0.03, 0.663 ±0.05 vs 0.872 ±0.08, P 〈 0.05）.
CONCLUSION： RXC has inhibitory effect on the replication of HBV.
出处
《世界华人消化杂志》
CAS
北大核心
2007年第4期394-398,共5页
World Chinese Journal of Digestology
