摘要
应用RAPD技术筛选与棱果沙棘性别相关的分子标记,对棱果沙棘雌雄株的基因组DNA进行混合分组分析(BSA),在194条随机引物中有50条引物能够在雌雄DNA反应池间形成多态性条带,应用这50条引物分别对棱果沙棘雌雄个体(雌雄个体各选取5个)进行RAPD分析,其中引物S10扩增得到1个约为1030bp的与雌性相关的RAPD标记。该标记的获得进一步表明棱果沙棘雌雄株间存在基因水平的差异,为棱果沙棘的性别研究提供分子依据。进一步利用该雌性特异位点设计出更加稳定的SCAR标记,可望用于棱果沙棘的早期性别的准确鉴别。
This article used the methods of random amplified polymorphic DNA （RAPD） with bulked segregant analysis（BSA） to identify markers that linked to sex determination in the dioecious Hippophae goniocarpa. DNA was extracted and two bulks were created, each containing equal amounts of DNA from ten individual of Hippophae goniocarpa. A total of 194 primers were screened. 50 primers produced clear patterns with at least one band that appeared to be polymorphic between male and female bulks. Sebsequently, 5 male and female individuals were analysed with those 50 primers, and only S10 （CTGCTGGGAC ） could generate a common 1 030 bp DNA fragment in all five female individuals and female pool but not in any male individuals. In the present study, the sex-linked RAPD marker, S10-1030, showed that there were different between female and male Hippophae goniocarpa on the level of DNA molecule and can be concluded that gender of Hippophae goniocarpa most likely is genetically determined. While the further research should focus on designing the more reliable molecular marker that based on the sex-determination gene, such as SCAR marker, for accurate and quick determination the gender of seedling in Hippophae goniocarpa.
出处
《植物研究》
CAS
CSCD
北大核心
2007年第1期73-76,共4页
Bulletin of Botanical Research
基金
国家自然科学基金(30570110
30270091)
西北师范大学重点学科经费
西北师范大学学生学术科研资助金资助项目
