摘要
研究了卟啉类光敏药物主要活性组分：原卟啉（ＰＰ）、血卟啉（ＨＰ）及双卟啉醚、酯混合物（ＤＨＥ）对Ｈｅｌａ细胞（宫颈癌）的光敏杀伤作用特性，首次发现ＰＰ对癌细胞杀伤力最大，是最有效的卟啉组分。进而研究了其作用的分子机制，采用激光喇曼光谱技术，研究了三种组分对溶菌酶空间结构的光敏损伤。结果表明，它们对溶菌酶主链和侧链结构的损伤明显不同：ＰＰ对蛋白质主链结构中的β—折叠，β—回折的光敏损伤明显强于ＨＰ和ＤＨＥ且不随浓度而变．
Characteristics of Photosensitive killing on main and active componentes PP(Protoporphyrin), HP (Hematoporphyrin) and DHE (Dihematoprophyrin ether and ester)of porphyrin's drug to Hela cells were studied. It was found for the first time that PP is the most effective componente in killing cancer cells. Then the molecular mechanisms of photosensitive damages of the three componentes to lysozyme were investigated by laser Raman spectroscopy. The results indicated that the damages of these componentes to main - chain and side - chain structures of lysozyme are considerably different: The damages of PP to β - sheet and β - turn of the main - chain were clearly stronger than those of HP and DHE and the results were found in a dose - independent way.
出处
《生物物理学报》
CAS
CSCD
北大核心
1996年第4期724-730,共7页
Acta Biophysica Sinica
基金
国家自然科学基金
关键词
卟啉类光敏药
癌细胞
溶菌酶
光敏杀伤特性
Main componentes of porphyrin' s drugs Cancer cells Lysozyme Photosensitization
