摘要
对灰轮丝链轮丝菌ZC60所产生的谷氨酰胺转胺酶采用(NH4)2SO4盐析、透析脱盐、冷冻浓缩和SephadexG-100凝胶过滤层析进行纯化,经SDS-PAGE检测,样品达到电泳级纯。对冷冻浓缩后得到的粗酶的酶学性质进行研究,得到该酶的最适反应温度为50℃,最适pH为7.0;酶的温度稳定范围为0～50℃,pH稳定范围为6.0～7.5;K+、Na+、Ca2+、Ba2+、Mg2+对MTG的活性几乎没有影响,而Pb2+,Cu2+抑制酶活。
The crude enzyme of transgulaminase from streptoverticillium griseoverticillatum was purified using saturation of ammonium sulfate, dialyzing. freeze concentrating and sepHadex G-100,The apparent purity was examined by SDS-PAGE. The properties of the crude enzyme were studied. The optimal temperature and pH were 50℃ and 7,0.respectively. The crude enzyme was stable below 50℃ and within pH 6.0-7.5. Metal ions of K＋,Na＋,Ca2＋,Ba2＋ and Mg2＋ had little effect on the activity of MTG. but Pb2＋ and Cu2＋ can inhibit the activity of the enzvme,
出处
《食品工业科技》
CAS
CSCD
北大核心
2007年第1期60-62,共3页
Science and Technology of Food Industry
基金
辽宁省科技厅科技攻关项目(2004205001)
辽宁省教育厅项目(2005369)
沈阳农业大学中
青年硕士导师项目资助
