期刊文献+

云南纳西族10个X-STR基因座遗传多态性研究 被引量:3

Genetic polymorphism of ten X-STR loci in naxi population
下载PDF
导出
摘要 为研究云南纳西族人群10个位于X染色体的短串联重复序列基因座及单倍型的遗传多态性,采用PCR扩增。变性聚丙烯酰胺凝胶电泳结合银染显色分型技术,对98名云南纳西族无关男性个体x染色体的10个STR基因座进行基因分型。结果显示。98名无关男性个体中。DXS7423、DXS7424、DXS6799、DXS7133、DXS6804、DXS8378、HPRTB、DXS7130、DXS7132和DXS6789分别检出4、7、6、3、6、5、5、7、6和8个等位基因。等位基因频率分布在0.0102(DXS7132、DXS6789)~0.7347(DXS7133)之间.由DXS8378与DXS7130基因座组成的单倍型共检出20种,由DXS6789、DXS6799和DXS7424基因座组成的单倍型共检出56种。单倍型多样性分别为0.8553和0.9649,说明所选的10个X—STR位点有较高的多态性信息,在基因组多样性研究、法医学个体识别、亲权鉴定中具有重要应用价值。 We studied the genetic polymorphism and haplotypes of ten short tandem repeats (STR) loci on the X chromosome in Yunnan Naxi population. Genotyping and detection of PCR products from the ten X-STRs were carried out on denaturing polyacrylamide gel electrophoresis followed by silver staining. The results revealed that among 98 males from Yunnan Naxi population, the number of alleles in the 10 loci (DXS7423, DXS7424, DXS6799, DXS7133, DXS6804, DXS8378, HPRTB, DXS7130, DXS7132 and DXS6789) were 4, 7, 6, 3, 6, 5, 5, 7, 6 and 8, respectively. Gene frequencies ranged from 0.0102 (DXS7132 and DXS6789) to 0.7347 (DXS7133). A total of 20 haplotypes were detected in DXS8378 and DXS7130 loci, and 56 haplotypes were detected in DXS6789, DXS6799 and DXS7424 loci. The haplotype diversity reached 0.8553 and 0.9649, respectively. In conclusion, the 10 X-STR loci are relatively abundant in polymorphic information for forensic identification, paternity testing and for genetic population studies.
出处 《遗传》 CAS CSCD 北大核心 2007年第2期180-184,共5页 Hereditas(Beijing)
基金 教育部科技基础资源数据平台建设项目"中华民族群体遗传资源数据整合共享平台(编号:505015)" 陕西省科技攻关项目(编号:2004K09-G12)~~
关键词 X染色体 短串联重复序列 遗传多态性 单倍型 中国纳西族 X-chromosome short tandem repeat genetic polymorphism haplotype Chinese Naxi population
  • 相关文献

参考文献13

二级参考文献64

  • 1吕德坚,刘秋玲.X染色体STR的法医学应用进展[J].中国法医学杂志,2002,17(4):252-255. 被引量:33
  • 2张修武,郭实士.应用PCR/SSO方法分析湖南籍汉族人HLA-DR,DQ,DP的DNA多态性[J].湖南医科大学学报,1994,19(1):7-10. 被引量:8
  • 3吕宝忠.多态信息量(PIC)等于杂合度吗?[J].遗传,1994,16(4):31-33. 被引量:15
  • 4于桂兰,孙逸平,徐林敏,高风云,李桂芹,郭玉红,邵惠心,沈晶晶.沈阳汉族人群HLA-DR、DQ、DP的DNA分型研究[J].中国免疫学杂志,1995,11(3):142-145. 被引量:18
  • 5[1]Hammond HA, Jin L, Caskey CT, et al. Evaluation of 13 short tandem repeat loci for use in personal identification appli- cations [J]. AJ Hum Genet, 1994, 55:175
  • 6[2]Edwards A, Cavitello A, Hammond HA, et al. DNA typing and genetic mapping with trimeric and tetrameric tandem repeats [J]. AJ HumGenet, 1991, 49:746
  • 7[3]Desmarais D, Zhong Y, Chakraborty R, et al. Development of a highly polymorphic STR marker for identity testing purposes at the human androgen receptor gene (HUMARA) [J].J Forensic Sci, 1998, 43 (5): 1046
  • 8[4]Kishida T,. Tarnaki Y. Japanese population data on X-chro mosomal STR locus AR [J]. Nippon Hoigaku Zasshi, 1997,51 (5): 376
  • 9[5]Heame CM,. Todd JA. Tetranucleofide repeat polymrphismat the HRPT Locus [J]. Nucl Acids Res, 1991, 19:5450
  • 10[6]Kishida T, Wang W, Fukuda M, et al. Duplex PCR of the Y-27H39 and HPRT loci with reference to Japanese population data on the HPRT locus [J]. Nippon Hoigaku Zasshi, 1997,51 (2): 67

共引文献94

同被引文献65

引证文献3

二级引证文献6

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部