摘要
用紫外线对3个甘蓝型油菜品系的离体小孢子进行照射0-60s。未经照射的对照和已照射的小孢子于NLN培养基进行培养,诱导胚状体。结果表明,照射20s和60s对胚状体的再生没有影响,照射40s则有促进作用。
The isolated microspores from 3 lines of Brassica napus were irradiated with ultraviolet （UV） light for 0 - 60 seconds and cultured in NLN medium to induce embryogenesis. The results showed that the treatment of 40s produced higher embryo yield than those of 0, 20 and 60s, but no significant difference was found among 4 treatments for embryo yield.
出处
《核农学报》
CAS
CSCD
北大核心
2007年第1期17-19,43,共4页
Journal of Nuclear Agricultural Sciences
基金
国家"863"项目(2004-AA241100)
国家"948"引进项目(2003-Q04)
创新团队项目(IRT0442)
关键词
甘蓝型油菜
紫外线
离体诱变
小孢子培养
Brassica napus
ultraviolet light radiation
Microspore culture
in vitro mutation
