摘要
目的:研究核因子-κB诱骗寡核苷酸(nuclear factor-κB decoy oligodeoxynucleotide,NF-κBdecoy ODN)对大鼠视网膜缺血再灌注损伤的保护作用。方法:设计、合成NF-κBdecoy ODN和错配NF-κBdecoyODN的核苷酸序列。采用升高眼内压的方法建立大鼠视网膜缺血再灌注模型。缺血前10min,玻璃体内注射NF-κB decoy ODN或错配NF-κB decoy ODN。再灌注24h后,观察视网膜的组织学变化,测定视网膜的髓过氧化物酶(myeloperoxidase,MPO)活力。检测缺血前10min和再灌注24h的闪光视网膜电图(flash electroretinography,F-ERG),计算其b波振幅恢复率。结果:缺血的大鼠视网膜再灌注24h后,视网膜明显充血、水肿,有许多白细胞浸润,神经节细胞和内核层细胞减少,且视网膜MPO活力增加(P<0.05)、b波振幅恢复率下降(P<0.05)。玻璃体注射NF-κB decoy ODN的大鼠,视网膜的损伤性组织学改变减轻,MPO活力降低,b波振幅恢复率增加。而玻璃体注射错配NF-κB decoy ODN则无此作用。结论:玻璃体注射NF-κB decoy ODN能有效抑制大鼠视网膜缺血再灌注损伤,保护视网膜功能。
AIM： To investigate the protective effects of NF-κB decoy ODN on retinal ischemia-reperfusion injury in rats.
METHODS： NF-κB decoy ODN and scrambled NF-κB decoy ODN were synthesized. Retinal ischemia was induced by increasing the intraocular pressure in rats. NF-κB decoy ODN or scrambled NF-κB decoy ODN was injected into the vitreal chamber at 10 minutes before retinal ischemia. The retinal histologic changes were observed and the activity of retinal myeloperoxidase （HPO） was analyzed. The recovery rate of F-ERG b wave amplitude was measured at 10 minutes before retinal ischemia and 24 hours after reperfusion.
RESULTS： The retinal hyperaemia and edema were observed and a lot of leukocytes infiltrated in retina with the ganglion cells and inner nuclear cells reducing at 24 hours after reperfusion. Moreover, the increasing of retinal HPO activity and decreasing of the recovery rate of b wave amplitude were found. Intravitreally injected NF-κB decoy ODN could ameliorate the histologic changes of retinal reperfusion injury, reduce retinal HPO activity and enhance the recovery rate of b wave amplitude. However, the protective effects on retinal reperfusion injury were not observed with scrambled AP-1 decoy ODN.
CONCLUSION： Intravitreally injected NF-κB decoy ODN could effectively ameliorate retinal ischemia-reperfusion injury and rescue retinal function in rats.
出处
《国际眼科杂志》
CAS
2007年第1期73-75,共3页
International Eye Science
