摘要
目的探讨人瘢痕成纤维细胞在体外持续培养时，其胶原合成能力的变化。方法体外持续培养人瘢痕成纤维细胞，检测不同代次细胞的大体形态、羟脯氨酸含量及超微结构的变化，并对比分析。结果第1、6、12、18代瘢痕成纤维细胞的羟脯氨酸（HPr）含量分别为（0．1460±0．0066）、（0．1020±0．0095）、（0．0700±0．0061）、（0．0540±0．0035）mg／L。随着体外培养细胞代次的增多，HPr呈下降趋势，并有相应的超微结构的变化。结论人瘢痕成纤维细胞在体外持续培养时，胶原合成能力逐渐下降，可能与其局部微环境的刺激因子有直接关系。第1-6代为最适实验细胞。
Objective To investigate the collagen synthesis ability of human hypertrophic-scarderived fibroblasts continuously cultured in vitro. Methods Human hypertrophic-scar-derived fibroblasts were cultured and passaged continuously. The changes in different passages were studied with gross pathohistological observation. The levels of HPr were detected and analyzed, and the ultra-structure of fibroblasts was observed by transmission electron microscopy. Results The HPr in 1stt, 6th, 12th and 18th passages was （0. 1460 ± 0.0066, 0. 1020± 0. 0095, 0.0700 ±0. 0061 and 0. 0540 ±0.0035 ） mg/L respectively. Under the condition of continuous culture in vitro, the levels of HPr in fibroblasts tended to decrease with the increase of generation, and the ultra-structure of fibroblasts changed accordingly. Conclusion Under the condition of continuous culture in vitro, the collagen synthesis ability of human hypertrophic-scar-derived fibroblasts is declined gradually, which is likely to be related directly to the changes of stimulators in the local microenvironment. The cells from 1st to 6th passages are more suitable for study.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2007年第2期161-162,共2页
Chinese Journal of Experimental Surgery
基金
广东省自然科学基金资助项目（04000570）
