摘要
以一株具有致病力的温和气单胞菌作为筛选宿主菌，从异育银鲫肠道中分离到一株蛭弧菌，暂命名为BDF-H16。通过光学显微镜、相差显微镜、电子显微镜对蛭弧菌BDF-H16进行形态观察，并研究了其部分生物学特性。结果表明：蛭弧菌BDF-H16为革兰氏阴性菌，杆形或弧杆形，端生一根鞭毛，菌体大小多为0．2μm-0．5μm×0．8μm—1.2μm；蛭弧菌BDF-H16对实验所选用的革兰氏阴性菌和部分革兰氏阳性菌均有裂解作用；以大肠杆菌为宿主菌，其最佳生长条件为宿主菌浓度6．75×10^9cfu／mL、pH7．0—7．5、温度28℃；在NaCl含量0．85％-5．00％的培养基中能够生长；恩诺沙星、诺氟沙星对其有抑制作用。
Bdellovibrio bacteria BDF - H16 was isolated from the gut of Carassius auratus gibelio with Aeromonas sobria. Its shape was observed by light microscopy, phase-contrast microscopy, electron microscopy and some of its biological characteristics were also studied. It was demonstrated that BDF- H16 was an gram-negative bacterium and had a bacilliform or arc bacilliform shape with a flagellum at one end. Its size was mostly 0. 2μm -0. 5μm ×0. 8μm - 1.2μm. It had a wide prey area and could lyso all tested gram -negative bacteria and some gram - positive bacteria. The best lysis conditions to Escherichia coli were 6. 75 × 10^9cfu/mL of prey bacteria concentration, pH7.0 - 7.5, 28℃. It could grow in the solid culture added 0. 85% -5. 00% NaCl and was inhibited by enrofloxacin and norfloxacin.
出处
《微生物学通报》
CAS
CSCD
北大核心
2007年第1期52-56,共5页
Microbiology China
基金
上海市重点学科建设资助项目(No.Y1101)
上海市教育委员会E-研究院建设资助项目(No.E03009)
关键词
异育银鲫
蛭弧菌
分离
生物学特性
Carassius auratus gibelio, Bdellovibrio bacteria, Isolate, Biological characteristics
