摘要
目的：建立一种保健食品中他达那非的反相液相色谱测定方法。方法：采用ODS色谱柱，0．02mol／L三乙胺：甲醇：乙腈（45：33：22）为流动相，流速1．0ml／min，二极管阵列检测器，检测波长217．4nm，标准曲线计算他达那非含量。结果：该方法他达那非在0．4～40μg／ml浓度范围内线性良好，相关系数0．9998，相对标准偏差为0．55％～0．71％，回收率为97．6％～105％，最低检出限为5ng。结论：该方法简便快速，准确可靠，可用于保健食品中他达那非含量的测定。
Objective：To set up a method of RP HPLC to determine the concent of tadalafil in health food. Methods：On a ODS column,using 0. 02 mol/L triethylamine/methanol/acetonitrile （45：33：22） as mobile phase. The flow rate was 1.0 ml/min. The detector was DAD. The decetive wavelength was set at 217.4 nm,and calibration trace method was used for quantification. Results：The linear range of calibration curve of tadalafil was 0. 4 -40 μg/mL The correlation coefficient was 0. 9998,and RSD was 0. 55% -0. 71%. The average recovery was 97. 6% -105%. The detection limit of tadalafil Was 5 rig. Conclusion：The method is simple,rapid,accurate and sensitiw It can be used as a quantitative analysing method for tadalafil in health food.
出处
《中国卫生检验杂志》
CAS
2007年第1期92-93,共2页
Chinese Journal of Health Laboratory Technology
关键词
反相高效液相色谱法
保健食品
他达那非
Reversed phase high performance liquid chromatography
Health food
Tadalafil
