摘要
目的探讨 KiSS-1、基质金属蛋白酶9(MMP-9)、核因子κB(NF-κB)p65蛋白3者在卵巢上皮性肿瘤组织中的表达及其相关性。方法采用免疫组化方法检测50份卵巢上皮性癌(卵巢癌)、20份卵巢交界性肿瘤、20份卵巢良性肿瘤和10份正常卵巢组织中 KiSS-1、MMP-9、NF-κBp65蛋白的表达,并分析其临床意义及3者间的相关性。结果 KiSS-1蛋白在卵巢癌组织中的阳性表达率(80%)明显高于良性肿瘤组织及正常卵巢组织(分别为35%、10%;P<0.05);在卵巢交界性肿瘤组织中阳性表达率(65%)明显高于正常卵巢组织(P<0.05)。在卵巢癌组织中,KiSS-1蛋白阳性表达率与淋巴结转移有关(P<0.05),与手术病理分期、病理类型及病理分级均无关(P>0.05);MMP-9蛋白阳性表达率与手术病理分期及淋巴结转移有关(P<0.05),而与病理类型及病理分级均无关(P>0.05);NF-κBp65蛋白阳性表达率与手术病理分期、病理分级及淋巴结转移有关(P<0.05),而与病理类型无关(P>0.05)。在卵巢癌组织中,KiSS-1与 MMP-9、NF-κBp65蛋白表达呈显著负相关关系(rs=-0.547,P<0.05;rs=-0.414,P<0.05);MMP-9与 NF-κBp65蛋白表达呈显著正相关关系(rs=0.695,P<0.05)。结论 KiSS-1基因可能对卵巢癌的转移起一定的抑制作用;KiSS-1基因可能通过抑制 MMP-9、NF-κB基因,从而发挥抑制卵巢癌转移的作用。
Objective To investigate the expression and correlation of KiSS-l, matrix metalloproteinase-9（ MMP-9 ）and nuclear factor（NF）-gBp65 proteins in primary epithelial ovarian tumors. Methods Expression of KiSS-1, MMP-9, NF-KBp65 proteins in primary ovarian epithelial tumors （malignant n = 50,berderline tumor n = 20,benign adenoma n =20, normal tissue n = 10） was evaluated by immunohistochemical staining. Results Expression of metastin protein in primary epithelial ovarian cancers was significantly higher than that in ovarian benign adenoma（ P 〈 0. 05 ） and normal tissues（ P 〈 0. 05 ）. Expression of metastin protein in ovarian borderline tumors was significantly higher than that in normal tissues（P 〈 0. 05 ）, Expression of metastin protein in ovarian cancer was significantly correlated with node metastasis （ P 〈 0. 05）. However, Metastin protein expression was not correlated with different histological classifications （P 〉0. 05）, differentiation grade（ P 〉0. 05） and International Federation of Gynecology and Obstetrics （FIGO） stage （P 〉 0. 05 ）. MMP-9 protein was positive in 68% （34/50） of the epithelial ovarian cancers, significantly higher than that in normal tissues （20%, 2/10; P 〈 0. 05 ）. NF-κBp65 protein was positive in 72% （36/50） of the epithelial ovarian cancers, significantly higher than that in ovarian benign adenoma（30% ,6/20; P 〈 0. 05 ） and normal tissues （ 10%, 1/10 ; P 〈 0. 05 ）, The expression of MMP-9 protein in epithelial ovarian cancer was significantly correlated with FIGO stage（P 〈 0. 05 ） and lymph node metastasis （P 〈0, 05）. However, MMP-9 protein expression was not correlated with different histological classifications（P 〉 0. 05 ） and differentiation grade （P 〉 0. 05 ）. The expression of NF-KBp65 protein in epithelial ovarian cancer was significantly correlated with FIGO stage （ P 〈 0. 05 ）, differentiation grade （ P 〈 0. 05 ） and lymph node metastasis（P 〈 0. 05 ）. However, NF-κBp65 protein expression was not correlated with different histological classifications （P 〉 0. 05 ）. There was obviously negative correlation between KiSS-1 and MMP-9 expression in ovarian cancer（rs = - 0. 547, P 〈 0. 05）, as well as between KISS-1 and NF-κBp65 expression in ovarian cancer（ rs = -0. 414, P 〈 0. 05 ）, while there was obviously positive correlation between MMP-9 and NF-κBp65 expression in ovarian cancer （ rs = 0. 695, P 〈 0.05）. Conclusion The results indicate that KISS-1 plays some role in suppression of the metastasis of ovarian epithelial cancers, which may be through inhibiting the expression of MMP-9 and NF-κBp65 .
出处
《中华妇产科杂志》
CAS
CSCD
北大核心
2007年第1期34-38,共5页
Chinese Journal of Obstetrics and Gynecology
基金
江西省卫生系统学术带头人计划(20045047)
