摘要
目的研究纤维连接蛋白1（FN1）基因表达与人纤维肉瘤肺转移的相关性。方法将HT1080细胞经裸鼠尾静脉注射接种，采集肿瘤浸润组与正常对照组肺组织RNA，合成cDNA，以含1176个人肿瘤相关基因的芯片行cDNA微阵列分析，以实时定量RT-PCR法测定两组FN1表达。利用激光捕获法采集实验组小鼠肿瘤浸润肺组织及周围组织RNA，分别以实时定量RT-PCR法测定FN1表达。结果肺转移组与对照组相比，表达差异基因31个，占2．3％，其中表达下调的基因占0．3％，表达上调的基因占2．3％，FN1表达明显上调。肿瘤浸润肺组织与周围组织相比，FN1表达明显上调。结论FN1基因高表达可能与人纤维肉瘤肺转移有关。
Objective To investigate the genes involved in pulmonary metastasis of human fibrosarcoma HT1080 cells in nude mice. Methods HT1080 cells were injected into the tail vein of BALB/ C nude mice. RNA samples were extracted from pulmonary metastatic tissues and normal control lung tissues, purified using Atlas Pure Total RNA Labeling System （Clonetech Laboratories）. cDNA probes labeled with ^32P were prepared and hybridized to a cDNA membrane constructed with spots of 1176 human cancer related genes and radioactivities on the membrane were measeured by BAS 5000. The mRNA expression of gene FN1 was determined by real time RT-PCR using TaqMan methods. Furthermore, cells with FN1 expression were localized and obtained in situ in pulmonary metastatic foci by laser captured micredissection, and the FN1 expression was quantitated by real time RT-PCR. Results Of the total 1176 genes, 27 genes （2.3%） revealed to be apparently up-regulated and 4 genes （0.3%） down-regulated. Real time RT-PCR analysis verified significant up-regulation of gene FN1. Laser captured microdissection/ real time RT-PCR analysis demonstrated up-regulated gene FN1 not in stroma cells but in tumor cell nests. Conclusion Gene FN1 expression in fibrosarcoma HT1080 cells may be involved in pulmonary metastasis.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
2007年第1期14-16,共3页
Chinese Journal of Oncology
关键词
人纤维肉瘤
肺肿瘤
肿瘤转移
FN1基因
Human fibrosarcoma
Lung neoplasms
Neoplasm metastasis
FN1 gene
