摘要
目的建立利福昔明含量和有关物质的HPLC测定法。方法使用Resolve C18柱(150mm×5.0mm,5μm);柱温40℃;流动相:甲醇∶乙腈∶0.05mol/L磷酸二氢钾溶液∶0.5mol/L柠檬酸溶液(15∶30∶26∶4);流速1ml/min;检测波长254nm;进样量20μl。结果利福昔明在浓度为0.2-0.05mg/ml范围内呈很好的线性关系,r=0.9999,重复性和日内精密度RSD分别为0.25%(n=5)和0.83%(n=6)。结论该方法简单、准确、专属性强,可用于利福昔明含量及有关物质的测定。
Objective To establish an HPLC method for the determination of content of rifaximin and its related substances. Methods A Resolve C18 column （150mm×5.0mm, 5μm） was used; the temperature of column： 40℃; mobile phase： methanol ： acetomitrile ： 0.05mgl/L potassium dihydrogen phosphate solution-and 0. 5mol/L citric acid solution （15 ： 30 ： 26 ： 4）; flow rate： 1.0ml/min; wavelength： 254nm; the injection volume was 20μl. Results The linear range for rifaximin was 0.2mg-0.05mg/ml （r=0. 9999）, repeatability and the inter-day precision RSD was 0.25% （n=5） and 0.83% （n=6）. Conclusion The method was convenient, specific, accurate and can be used for determinatim of rifaximin and its related s ubstances.
出处
《中国抗生素杂志》
CAS
CSCD
北大核心
2007年第2期89-90,103,共3页
Chinese Journal of Antibiotics
