摘要
目的探讨洛伐他汀(LOV)对人类肾小球系膜细胞(HMC)增殖及细胞周期的影响。方法人类系膜细胞常规培养。实验分为3组:正常对照组(10%胎牛血清)、洛伐他汀组(10μmol/LLOV)、甲羟戊酸(MVA)干预组(10μmol/LLOV+100μmol/LMVA)。每组分别在干预因素作用24,48h时用MTT法检测HMC在490nm波长处的光吸收(A490nm)值,观察LOV对HMC增殖的影响。用流式细胞仪测定HMC的细胞周期G1(DNA合成前期)与S期(DNA合成期)的比率,观察LOV对HMC周期进展的影响。结果MTT法结果显示24,48hLOV抑制HMC增殖,A490nm值分别为0.108±0.010和0.216±0.009,与正常组比较P<0.05。100μmol/LMVA可逆转其作用。细胞周期结果显示24,48hLOV抑制HMC细胞周期进展,S/G1比例分别为0.150±0.009和0.301±0.010,与正常组比较P<0.05。100μmol/LMVA可逆转其作用。结论LOV抑制HMC增殖,阻止细胞周期进展,MVA可逆转其抑制作用。
Objective To study the effects and mechanism of lovastatin （LOV） on proliferation and cell cycle of cultured human glomerular mesangial cells （HMC） in vitro. Methods Human glomerular mesangial cells were conventionally cultured. The experiment were divided into 3 groups： normal control group（ 10% FCS） ,LOV group （ 10 μmol/L LOV） and MVA intervention group （10 μmol/L LOV ＋ 100 μmol/L MVA）. HMC proliferation was determined with MTT colormetric method in each group at hours 24,48 after intervention. HMC cell cycles were determined by flow cytometry. Results The MTT results showed LOV inhibited HMC proliferation. Compared with control group, the values of A490 nm in LOV group were 0. 108 ± 0.010,0. 216 ± 0. 009 at hours 24 and 48, respectively （ P〈0.05）.But concomitant addition of 100 μmol/L MVA could reverse its effect. The consequence of cell cycles showed that LOV induced G1/S transition arrest. Compared with control group, values of S/G1 were 0. 150 ± 0. 009 and 0. 301 ± 0. 010 at hours 24 and 48 in LOV group（ P 〈 0.05）. But concomitant addition of 100μmol/L MVA could reverse the effect. Conclusion LOV can inhibit the HMC proliferation and induce the G1/S transition arrest, while concomitant addition of 100 μmol/ L mevalonic acid can reverse its effects.
出处
《山西医科大学学报》
CAS
2007年第2期126-129,共4页
Journal of Shanxi Medical University
基金
山西省科技攻关基金资助项目(2006031089-2)
关键词
甲羟戊酸
洛伐他汀
肾小球膜
细胞周期
mevalonic acid
lovastatin
glomerular mesangium
cell cycle
