摘要
[目的]观察阿托伐他汀(atrovastatin)对大鼠pMCAO(permanent occlusion of middle cerebral artery)模型脑eNOS(endothelial nitric oxide synthase)的表达及其表达规律的影响,探讨其具有脑保护作用的基础。[方法]采用线栓法制备pMCAO模型,并将试验动物随机分组:假手术组(A组),pMCAO组(B组),pMCAO+阿托伐他汀组(C组),pMCAO+提前给阿托伐他汀组(D组),每组再分为3个小组:2,3,7d组。比较各组Bederson神经行为学评分、梗死体积,以及eNOS的表达。[结果]神经行为学评分,梗死后3d和7d,B、C两组之间差异有统计学意义(P﹤0.05);梗死体积测定,在2、3、7d,B组与C组、D组比较差异有统计学意义(P﹤0.05);C组与D组比较差异无统计学意义(P﹥0.05)。eNOS阳性表达2、3、7d,各组之间比较差异有统计学意义(P﹥0.05)。[结论]阿托伐他汀可提高大鼠的神经行为学评分,减少梗死体积,增加eNOS的表达,促使eNOS表达的增加是阿托伐他汀具有脑保护作用的基础。
[Objective] To investigate eNOS expression and change tendency under treatment with atrovastains in rats with permanent occlusion of middle cerebral artery （pMCAO）, and to explore the basis of its neuroprotective effects. [ Mothods] The pMCAO models were established with thread embolism of middle cerebral artery. The rats were divided into 4 groups randomly： sham operation group （A group）, pMCAO group （B group）, pMCAO plus atrovastatin group （C group）, the group of pMCAO plus atrovastatin given in advance, and each group was further divided into 3 subgroups： 2d group, 3 d group, and 7 d group. Then they were evaluated in Bederson neurological status, infarct volume, and eNOS expression level In Bederson score, group B performed significant difference with group C at 3d and 7d post pMCAO; In infarct volume, group B performed significant difference with group C and group D at 2 d, 3 d, and 7 d post pMCAO, but there was no difference between group C and group D. With regard to eNOS, at 2 d, 3 d and 7 d post pMCAO, group B performed significant difference with group C and group D. [Conclusions] Atrovastatin can improve the neurologie deficit, decrease infarct volume, and increase eNOS expression, which make atrovastatin have neuroproteetive effects.
出处
《现代预防医学》
CAS
北大核心
2007年第3期481-483,492,共4页
Modern Preventive Medicine
