摘要
蝴蝶兰叶片组织在无菌条件下接种于添加1.8 mg.L-16-BA和1.5 mg.L-1NAA的MS培养基(细胞诱导分生培养基)中,培养7 d,叶片细胞逐渐增殖培养生成芽苗;将芽苗切割分离接种于添加1.0 mg.L-16-BA和1.0mg.L-1NAA的MS培养基(芽苗增殖培养基)中,无菌培养9 d后,蝴蝶兰芽苗诱导生成幼苗;将蝴蝶兰幼苗转接入添加激素0.1 mg.L-1NAA的MS培养基(生根培养基)中,培养15 d后,幼苗诱导生根,形成完整的蝴蝶兰小植株。
The explants of Phalaenopsis spp. lamina were cultivated in the experiment. Results showed that the buds could be directly induced from lamina inoculated on MS＋ 1.8 mg · L^-1 6-BA＋ 1.5 mg · L^-1 NAA medium for 7 d. The buds were inoculated on MS＋1. 0 mg · L^-1 6-BA＋1. 0 mg · L^-1 NAA medium and formed many clumped sprouts for 9 d and then differentiated gradually into plantlets. The excised plantlets could produce root after inoculated on MS medium with 0.1 mg · L^-1 NAA for 15 d and developed into normal plants successfully.
出处
《化学与生物工程》
CAS
2007年第2期58-59,共2页
Chemistry & Bioengineering
基金
襄樊市科技攻关计划项目(2006GG2C21)
关键词
蝴蝶兰
叶片组织
快速繁殖
工艺
方法
Phalaenopsis spp.
lamina tissue
ectogenesis
technique
method
