摘要
研究了NAA,IBA和KT 3种组织培养中常用的植物生长调节物质在溶液中的化学稳定性,结果表明:3种植物生长调节物质在水溶液中长期保存均易造成分解,其母液可准确使用的保存时间为不超过10 d;温度和光照条件对NAA在水溶液中的稳定性无明显影响;在需要较长时间保存使用时,IBA母液必须遮光保存,KT母液必须低温遮光保存;以95%酒精为溶剂可以大大提高KT和IBA在母液中的稳定性,对于需要较长时间保存使用的KT和IBA母液应以95%的酒精为溶剂配制.
The chemical stability of 3 categories of most commonly applied plant growth regulators solutions in plant tissue culture, i. e. , NAA, IBA and KT solutions under different storage conditions was studied. The results showed that ： In any cases, water solutions of the 3 categories of plant growth regulators would decompose gradually. So, for the accuracy of the experiments, the water solutions can be stored for not more than 10 days. The temperature and light conditions had no affect on the stability of NAA water solution. But the mother solution of IBA must be stored in darkness to keep the stability, and the mother solution of KT must be stored under conditions of low temperature and in darkness. 95% of alcohol solvent could greatly improve the stability of KT and IBA mother solutions compared with water solvent. Therefore, 95 % of alcohol ought be applied as solvent to prepare mother solutions for KT and IBA plant growth regulators for a longer time storage.
出处
《西南林学院学报》
2006年第5期10-12,共3页
Journal of Southwest Forestry College
基金
陕西省自然科学基金资助项目(2004C126)
