摘要
目的:探讨基质金属蛋白酶-9与急性脊髓损伤后脊髓水肿的关系。方法:健康雄性SD大鼠70只,随机分为对照组和损伤组。对照组大鼠10只,仅做椎板切除术,术后6h取材;损伤组采用改良Allen法制作T8～T9节段脊髓损伤模型,分别于伤后6h、12h、1d、3d、5d、7d(每时间点10只)处死取材。采用干湿重法测定脊髓含水量,免疫组化检测基质金属蛋白酶-9的表达。结果:急性脊髓损伤后6h,损伤组脊髓含水量较对照组增多,3～5d时达到高峰。基质金属蛋白酶-9在对照组未见表达,而损伤组在损伤后6h表达增加,并在1～3d达到高峰,损伤后7d仍有表达。相关性分析显示基质金属蛋白酶-9的表达与脊髓含水量呈正相关。结论:急性脊髓损伤后,基质金属蛋白酶-9的表达与脊髓水肿的形成有关,但两者达到高峰的时间不完全一致。
Objective：To investigate the relationship between the early expression of matrix metalloproteinase-9 （MMP-9） and spinal edema after actue spinal cord injury.Method：70 rats were randomized into two groups,the sham-operated group and the injuried group.The 10 rats of sham-operated group were performed laminectomy alone.The models of spinal cord injury were introduced with Allen＇s way by a 10g×2.5cm impact on the posterior T8-T9 spinal cord.Rats were sacrificed at 6h, 12h, 1d,3d,5d,7d after injury.The water content of injuried spinal cord was measured by dry-wet weight method and the expression of MMP-9 was performed by immunohistochemical staining.Result：The water content of injuried spinal cord increased and reached the maximum at 3-5d.The expression of MMP-9 increased at 6h after spinal cord injury and reached peak in 1- 3d.The expression of matrix metalloproteinase-9 wasn＇t found in the sham-operated group.The data showed that the water content of spinal cord was positively correlated with the level of MMP-9.Conclusion：Afier acute spinal cord injury,the early spinal edema is related to the level of MMP-9,but the time of their reaching peak isn＇t absolutely consistent.
出处
《中国脊柱脊髓杂志》
CAS
CSCD
2007年第1期59-61,I0002,共4页
Chinese Journal of Spine and Spinal Cord
