摘要
利用转基因技术培育抗旱杨树新品种是改善干旱地区生态环境的有效途径之一.该研究对已整合外源SacB基因的银腺杂种杨株系进行半定量RT-PCR和温室水分胁迫试验.研究结果表明,在所测定的7个转基因株系中,外源SacB基因均成功转录mRNA;转基因株系叶片中积累了SacB基因表达产物(果聚糖);在干旱胁迫条件下,转基因株系的生长量、生物量和叶片含水量明显高于对照.相关分析表明转基因株系生长量、生物量和叶片含水量与果聚糖积累量呈极显著正相关,说明SacB基因的导入提高了转基因杨树对水分胁迫的抗性.
The production of drought tolerant transgenic poplars is one of the effective ways to, improve the ecological environment of arid regions. In this study, the expression of Sac B gene in transgenic lines of Populus alba × P. glandulosa was analyzed by RT-PCR and the drought tolerance of those transgenic lines was evaluated by water stress trial in greenhouse. The results showed that the transcription of Sac B was detected in all transgenic lines and the fructan accumulated in the leaves of those plants. The growth, biomass and leaf water content of some transgenic lines were significant higher than those of the control under water stress. The correlation analysis indicates that the concentration of fructan in leaves of transgenic lines is most significant positively related to growth, biomass and leaf water content. The results also show that the expression of Sac B gene in transgenic poplar enhances its resistance to water stress.
出处
《北京林业大学学报》
CAS
CSCD
北大核心
2007年第2期1-6,共6页
Journal of Beijing Forestry University
基金
"863"国家高新技术研究发展计划(2006AA100109)
关键词
SAC
B基因
银腺杂种杨
转基因
抗旱性
Sac B gene, Populus alba ×P. glandulosa, transgene, drought tolerance
