摘要
目的:探讨玉屏风散中黄芪甲苷最佳提取工艺及黄芪甲苷的定性与定量测定方法,以及玉屏风散总提取物的抗炎作用。方法:应用薄层色谱法与紫外分光光度法对黄芪甲苷进行鉴别和含量测定,从而确定玉屏风散总提取物的最佳制备工艺;采用小鼠耳肿胀法对玉屏风散总提取物的抗炎作用进行评价。结果:确定80%乙醇回流提取为玉屏风散中黄芪甲苷的提取工艺;其提取物对二甲苯诱导的小鼠耳肿胀有明显抑制作用。结论:玉屏风散中黄芪甲苷的提取工艺及质量控制方法达到医药制剂的要求,其提取物对二甲苯诱导的小鼠耳肿胀有明显抑制作用,提示其有较强的抗炎作用。
OBJECTIVE To discuss the preparation techniques and content determination method of total glycosides of Yu Pingfeng powders and to evaluate the anti - inflammatory effect of Yu Pingfeng powders. METHODS Thin layer chromatography （ TLC ） and UV were used to determinate Asernestiosode A of Astragalus and select the optimum technology to prepare Yu Pingfeng powders. Anti - inflammatory activity of Yu Pingfeng powders was investigated using edematous swelling model in mice. RESULTS The optimum technology was reflux extraction with the 80% ethanol. Yu Pingfeng powders had a significantly inhibitory effect on edematous swelling of ears in mice. CONCLUSION Yu Pingfeng powders have obvious anti - inflammatory activity.
出处
《天津药学》
2007年第1期31-32,55,共3页
Tianjin Pharmacy
关键词
玉屏风散
黄芪甲苷
提取工艺
定性定量分析
抗炎作用
Yu Pingfeng Powders, Astragaloside, extraction technology, qualitative and quantitative analysis, Anti - inflammatory
