摘要
目的：观察大肠杆菌脂多糖（1ipopolysaccharide，LPS）刺激后，皮肤成纤维细胞数量和增殖周期的变化规律。方法：体外培养正常人皮肤成纤维细胞，加入不同浓度（0．005—1．0μg／ml）的大肠杆菌LPS（E.coli055：B5），通过细胞计数法连续观察1～9d细胞数量的变化；于LPS加入后第6天细胞处于对数生长期时，采用流式细胞术观察细胞增殖周期。结果：LPS刺激浓度在0．005～0．1μg／ml时，细胞数量和S期细胞比例随着刺激浓度增加而增高，且呈浓度依赖性；当LPS浓度为0．5μg／ml时，上述作用开始降低，但仍高于对照组；当浓度达到1．0μg／ml时，细胞数量和S期细胞比例均低于对照组。结论：在一定浓度范围内LPS促进皮肤成纤维细胞增殖，而过高浓度LPS则呈现抑制效应。
Objective：To observe the influence of lipopolysaccharide（LPS） on the proliferation and cell cycle of normal human skin fibroblasts. Methods： Purified dermal fibroblasts were exposed to different concentrations of LPS （0. 005-1.0μg/ml） from E. coli. Then cells were＇counted on a daily basis from day 1 to day 9 under inverted phase contrast microscope after the administration of LPS, and the cell cycle of fibroblasts at logarithmic stage at day 6 after LPS administration was assayed with flow cytometry. Results：The amount and the percentage of S phase cells in cell cycle of normal human skin fibroblasts increased when challenged with LPS at the concentrations of 0. 005-0.1μg/ml, and the increase showed a concentration dependent manner. However, when the concentration of LPS was 0.5 μg/ml, cell amounts and the percentage of S phase cells began to decrease, but still higher than the control group. When LPS concentration reached 1.0μg,/ml, both cell amounts and percentage of S phase cells were lower than the control group. Conclusion： LPS promotes the proliferation of normal human skin fibroblasts within a certain range of low concentrations, while high concentration of LPS inhibits the proliferation of normal human skin fibroblasts.
出处
《军医进修学院学报》
CAS
北大核心
2007年第1期1-3,共3页
Academic Journal of Pla Postgraduate Medical School
基金
国家自然科学基金资助项目(30371467)
关键词
脂多糖类
成纤维细胞
细胞增殖
细胞周期
lipopolysaccharides
fibroblasts
cell proliferation
cell cycle
