摘要
目的:用荧光原位杂交方法(FISH)观察移植肾中血管内皮细胞被受体内皮细胞替代(内皮嵌合)的现象,并分析内皮嵌合对肾脏移植物预后的影响。方法:移植后2周到4个月男性供者、女性受者的移植肾穿刺活检标本34例,选择Y染色体长臂Yq12区域(异染色质区)DNA片段作为探针,同时选择X染色体着丝粒区(α卫星DNA)探针作为对照,通过在石蜡切片标本上进行间期细胞双色荧光原位杂交,分析血管内皮细胞被受体替代的现象,分析其对移植肾预后的影响。结果:肾脏移植物中血管内皮细胞嵌合现象较普遍存在,内皮细胞的分布呈灶状,供者内皮细胞和受体内皮细胞可相邻存在。血管内皮细胞嵌合的发生与否移植肾1年血清肌酐值分别为(115.88±25.06)μmol/L,(118.16±18.85)μmol/L,差异无显著性意义(P>0.05)。结论:肾脏移植物中血管内皮细胞可以被受者来源的内皮细胞所替代,对移植后1年的肾功能无明显影响,内皮嵌合对移植肾的长期存活的影响有待进一步观察。
Objective ：To investigate the presence of endothelial chimerism and its relationship with prognosis of renal allograft. Methods：We studied biopsy samples from 34 renal transplants of female recipients who received the kidney from a male donor for the presence of endothelial cells of recipient origin. Formalin-fixed, paraffin-embedded tissue sections of renal biopsy samples were examined by fluorescence in situ hybridization for the presence of endothelial cells containing two X-chromosomes using a biotinylated Y-chromosome probe and digoxigenin labeled X-chromosome probe. Results：The FISH methods did identify endothelial cells of recipient origin. The difference of serum creatinine post one year of transplantation in the group of presence or absence of endothelial chimerism was not statistically significant （ 115.88 ±25.06） mol/L vs （ 118.16 ± 18.85） mol/L（P 〉0.05）. Conclusion：The impact of endothelial chimerism on renal allograft long time survival needs continue follow-up visits.
出处
《军医进修学院学报》
CAS
北大核心
2007年第1期4-6,共3页
Academic Journal of Pla Postgraduate Medical School
基金
首都医学发展科研基金(2005-3093)
关键词
原位杂交
荧光
肾移植
内皮细胞
移植嵌合体
in situ hybridization, fluorescence
kidney transplantation
endothelium cells
transplantation chimerism
