摘要
微小生命个体的冷冻复活研究,可以为今后实现极具挑战性的动物个体乃至人体的冷冻复活提供实验依据。对黑背蚂蚁进行了冷冻(-15℃左右)复活研究,试验探讨了蚂蚁复活率与冷冻速率、时间、温度水平的关系,以及喂饲葡萄糖对蚂蚁复活率的干预作用。结果显示:蚂蚁冷冻后的复活率可能与降温速度呈正比,至少快速降温不会降低蚂蚁的复活率;冷冻时间越长,温度越低,蚂蚁复活率越低;喂饲葡萄糖则可以增加蚂蚁的抗冷冻损伤能力。
Tests on the freezing revival of a micro biological body may provide experimental evidences to support future scientific efforts in freezing and surviving an animal or even a human being. In this study, revival experiments of ants subject to cooling around -15℃ were performed. Correlations between the ant revival ratio and the freezing rate, the time and temperature levels were investigated. Particularly, the influence of in vivo feeding with glucose on the ant revival ratio was also tested. It is found that the revival ratio of the ants under freezing around -15℃ is directly proportional to the freezing rate, at least, a rapid freezing will not lower the revival ratio. The longer freezing time and the lower freezing temperature, the lower revival ratio will be. If feeding with glucose, the capability for the ant to withstand the freezing injury may be improved.
出处
《科技导报》
CAS
CSCD
2007年第5期34-37,共4页
Science & Technology Review
基金
国家自然科学基金项目(50325622
50436030)
关键词
活体保存
低温生物
冷冻复活
蚂蚁冻存
抗冻剂活体加载
温度测试
preservation of biological body
cryobiology
freezing revival
ant cryopreservation
in vivo loading of anti freeze agent
temperature measurement
