摘要
目的:探讨增殖细胞核抗原(PCNA)和P27蛋白在前列腺癌中的表达及临床意义。方法:应用免疫组化S-P方法检测30例前列腺增生和46例前列腺癌中的PCNA、P27蛋白的表达。结果:前列腺增生组织中的PCNA强阳性表达率为6.7%,前列腺癌强阳性表达率为82.6%,两者比较有显著的差异(P<0.01)。PCNA的强阳性表达率与前列腺癌的分化程度呈负相关(P<0.05〉,与临床分期呈正相关(P<0.05)。前列腺增生组织中P27蛋白的强阳性表达率为70%,前列腺癌中的表达率为17.4%,两者比较有显著差异(P<0.01),P27蛋白的表达与前列腺癌组的分化程度呈正相关(P<0.05〉,与临床分期呈负相关(P<0.05),在前列腺增生组织及前列腺癌中PCNA与P27蛋白表达呈负相关(P<0.05)。结论:同时检测P27、PCNA对前列腺癌的预测,恶性程度的判定,预后的估计有重要指导意义。
OBJECTIVE：To study the expression of PCNA and P27 protein in patients with prostate carcinoma and to study its clinical significance. METHODS： The expressions PCNA and P27 in 30 patients with hyperplasia of prostate gland and 46 with prostate carcinoma were detected by S - P immunohistochemical method. RESULTS： Strong positive expression rates of PCNA in patients with hyperplasia of prostate gland and in those with prostate carcinoma were 6.7 % and 82.6 %, respectively, and there were significant differences between 2 groups （ P 〈 0.01 ）. The strong positive expression of PCNA showed a negative correlation with differentiation degree of prostatic carcinoma （ P 〈 0.05） but a positive correlation with its clinical stage （ P 〈 0.05）. Strong positive expression rates of P27 in patients with hyperplasia of prostate were 70 %, and 17.4% in those with prostate carcinoma, and there were significant differences between 2 groups （P 〈 0.01 ）. The expression of P27 showed a positive correlation with differentiation degree of prostatic carcinoma （ P 〈 0.05）, but a negative correlation with clinical stage （ P 〈 0.05）. A negative correlation was found between P27 and PCNA expressions in both hyperplasia of prostate gland and prostate carcinoma （ P 〈 0.05）. CONCLUSION ： It is of great significance to determine P27 and PCNA for the diagnosis, malignant grading and prognostic estimation of prostate carcinoma.
出处
《中国医院用药评价与分析》
2007年第1期50-52,共3页
Evaluation and Analysis of Drug-use in Hospitals of China
关键词
增殖细胞核抗原
P27蛋白
前列腺癌
免疫组化
proliferating cell nuclear antigen （PCNA）
p27protein
prostate carcinoma
immunohistochemistry
