摘要
报道细胞分裂素和硝酸铵对欧洲云杉不定芽伸长过程的影响。采用5年生的欧洲云杉1个无性系不定芽丛来研究玉米素和硝酸铵对不定芽生长的影响。试验3种诱导类型(玉米素浓度分别为6·84、13·68、20·52μmol·L-1,激动素浓度0·69μmol·L-1,培养4周),玉米素最高浓度处理诱导的伸长芽数量最多,在4个无生长调节剂继代培养过程中,加入了1个硝酸铵浓度逐渐增加的继代培养过程。经不含生长调节剂的低浓度的硝酸铵(2·5mmol·L-1)培养基的继代培养后转入含有细胞分裂素的诱导培养基,下一个继代培养基补充的硝酸铵浓度分别为2·5、7·5、12·5、17·5mmol·L-1,8周(2个继代培养)后转入含有硝酸铵浓度为2·5mmol·L-1的培养基培养。硝酸铵浓度为7·5mmol·L-1的处理可形成数量最多的不定芽,这对于采用不同浓度的玉米素进行诱导的处理结果是一致的,但随着硝酸铵的浓度增至12·5mmol·L-1和17·5mmol·L-1时则不定芽的数量减少。欧洲云杉的胚轴外植体经1次或多次诱导后,细胞分裂素(BA、激动素、玉米素、2iP)促进了不定芽的分化和发育,其中BA由于可诱导产生大量的不定芽而成为最有效的细胞分裂素。玉米素虽然诱导不定芽相对弱一些,却在BA多次诱导后可促进芽的伸长。这对获得具有形成不定芽能力并可扩繁的芽丛非常重要。
The capability of different cytokinins （BA, kinetin, zeatin, 2iP） to stimulate the induction and development of adventitious buds was determined after single and repeated induction steps with hypoeotyl explants. BA was the most effective cytokinin as far as it concerned the number of induced adventitious buds. Zeatin, although less effective for this pattern, promoted the elongation of buds even after repeated induction cycles contrary to BA. This is important to get propagating bud clusters with the ability to form elongating shoots. A five-year-old propagating clone line of adventitious bud clusters was used to study the influence of zeatin and ammonium nitrate on the shoot elongation. Three induction variants （6.84, 13.68, 20.52 μmol·L^-1 zeatin, each with 0.69μmol·L^-1 kinetin for 4 weeks） were tested. The highest zeatin concentration induced the largest number of elongating buds. The influence of one subculture step with an enhanced ammonium nitrate concentration during the growth regulator free shoot development period （4 subcultures） was tested. A subculture on a medium free of growth regulators with a low ammonium nitrate concentration （2.5 mmol·L^-1 ） followed the cytokinin containing induction medium. The next subculture medium was supplemented with 2.5, 7.5, 12.5 and 17.5mmol·L^-1 ammonium nitrate. An 8-week period （2 subcultures） followed with 2.5 mmol·L^-11 ammonium nitrate. The treatment with 7.5mmol·L^-1 ammonium nitrate formed the highest number of elongating buds. This was identical for all zeatin concentrations used for induction. Increased ammonium nitrate concentrations during this treatment （12.5 mmol·L^-1 and 17.5 mmol·L^-1） decreased the number of elongating buds.
出处
《林业科学》
EI
CAS
CSCD
北大核心
2007年第1期28-34,共7页
Scientia Silvae Sinicae
关键词
欧洲云杉
不定芽
细胞分裂素
硝酸铵
Norway Spruce （Picea abies）
adventitious buds
cytokinin
ammonium nitrate
