摘要
目的建立香草醛-冰醋酸体系分光光度法测定毛老虎中总三萜酸的方法。方法利用在高氯酸的条件下香草醛-冰醋酸与三萜酸的反应测定总三萜酸。结果总三萜酸在2．53～13．14μg／ml呈良好的线，陛关系，测定样品回收率为99.07％，结论该法简便、快速、灵敏。
Objective To develop the determination method of total triterpenoids in vanillin-glacial acetic acid system by spectrophotometry. Methods Vanillin was used as colour developing reagent in the presence of perchloric acid. Results The calibration curve of total triterpenoids had good linear relationship in the range of 2.53 - 13, 14 μg/ml. The average recovery, was 99.07%. Conclusion The method is simple , easy and reproducible .
出处
《时珍国医国药》
CAS
CSCD
北大核心
2007年第2期297-298,共2页
Lishizhen Medicine and Materia Medica Research
基金
广西科技攻关资助项目(No.0480005)
关键词
毛老虎
分光光度法
香草醛
Hyptis suaveolens （ L. ） Piot
Spectrophotometry
Vanillin
