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银杏叶提取物诱导血红素氧合酶-1表达抗缺血再灌注损伤后心肌细胞凋亡的研究 被引量:4

Study of Ginkgo biloba Extract Protect against Cardiomyocyte Apoptosis from Ischemia Reperfusion Injury by Inducing HO-1 Expression
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摘要 目的探讨银杏叶提取物(EGb761)诱导血红素氧合酶-1(HO-1)表达抗缺血再灌注损伤(IRI)后心肌细胞凋亡的效果及其机理。方法48只兔随机均分为单纯IRI组、EGb761组、EGb761+ZnPPⅨ组,分别预先给予等容积的生理盐水,EGb761,EGb761+ZnPPⅨ腹腔内注入。24 h后,每组任选8只处死,检测心肌细胞内HO-1表达及活性,余兔结扎冠脉左前降支使心肌缺血40 min,再灌注4 h,取缺血心肌检测中性粒细胞浸润、丙二醛(MDA)含量、Bax蛋白与Bcl-2蛋白表达及心肌细胞凋亡指数(AI)。结果与单纯IRI组及EGb761+ZnPPⅨ组比较,EGb761组HO-1表达及活性均明显升高,中性粒细胞浸润及MDA含量明显降低,Bax增加而Bcl-2降低,心肌细胞AI显著减少(均为P<0.01)。与单纯IRI组比较,EGb761+ZnPPⅨ组HO-1表达增加但活性降低(P<0.05),MDA含量明显升高(P<0.05),其余指标均无显著差异。结论EGb761能明显诱导心肌细胞内HO-1表达,并通过HO-1的抗炎、抗氧化等作用下调Bax表达,上调Bcl-2表达,从而显著抑制IR损伤后心肌细胞凋亡。 Objective To explore the protective effects and mechanism of heme oxygenase - 1 ( HO - 1 ) expression induced by extract of Ginkgo biloba( EGb761 ) against cardiomyocyte apoptosis from ischemia reperfusion injury(IRI). Methods 48 rabbits were randomly divided into simple IR group, EGb761 group and EGb761 + ZnPPⅨ group. Each rabbit in different groups was intrapefitoneal injected with saline(5ml), EGb761 (10mg/kg), EGb761 (10mg/kg) + ZnPPⅨ (7.5mg/kg) respectively. 24 hours later, 8 rabbits in each group were killed to measure myocardial HO - 1 expression and HO - 1 activity. Other rabbits were subjected for 40min of myocardial ischemia by ligation of left anterior descending coronary artery followed by 4h of reperfusion. The neutrophil infiltration, myocardial malonaldehyde(MDA) content, Bax and Bcl - 2 protein expression, cardiomyocyte apoptosis index(AI) in ischemia areas were detected. Results Campared with simple IRI group and EGb761 + ZnPPⅨ group, the expression and activity of myocardial HO - 1 increased significantly, the neutrophil infiltration and MDA content decreased. Bax expression increased and AI and Bcl- 2 level decreased in EGb761 group (P 〈 0.01 respectively). Compared with simple IRI group, EGb761 + ZnPPⅨ group showed higher myocardial HO -1 expression but lower HO -1 activity (P 〈0.01 ), while the MDA content increased ( P 〈 0.05 ), but the neutrophil infiltration, Bax and Bcl - 2 expression and AI showed no significant difference. Conclusion EGb761 could induce HO - 1 overexpression, through its antiinflammatory and antioxidative action to upregulate Bcl -2 and downregulate Bax, thus protect against cardiomyocyte apoptosis from IRI.
出处 《时珍国医国药》 CAS CSCD 北大核心 2007年第2期362-364,共3页 Lishizhen Medicine and Materia Medica Research
关键词 银杏叶提取物 血红素氧合酶-1 再灌注损伤 凋亡 Extract of Ginkgo biloba Heine oxygenase - 1 Reprfusion injury Apoptosis
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参考文献9

  • 1Kusmic C,Basta G,Lazzcrini G,et al.The effect of Ginkgo biloba in isolated ischemic/reperfused rat heart:a link between vitamin E preservation and prostaglandin biosynthesis[J].J Cardiovasc Pharmacol.2004,44(3):356.
  • 2Chen JX,Zeng H,Chen X,et al.Induction of heme-oxygenase-1 by Ginkgo biloba extract but not its terpenoids partially mediated its protective effect against lysophosphatidylcholine-induced damage[J].Pharmacol Res,2001,43(1):63.
  • 3Vulapalli SR,Chen Z,Chua BH,et al.Cardioselective overexpression of HO-1 prevents I/R-induced cardiac dysfunction and apoptosis[J].Am J Physiol Heart Circ Physiol.2002,283 (2):H688.
  • 4何洁,张敏,刘俊昌,吴立玲,伍贻经.血红素氧化酶-1在离体大鼠心肌缺血预处理中的作用[J].中国病理生理杂志,2001,17(10):932-934. 被引量:13
  • 5Mullane KM,Kraemer R,Smith B.Myloperoxidase activity as a quantitative assesment of neutrophil infiltration into ischemic myocardium[J].J Pharmacol Methods,1985,14(3):157.
  • 6von Harsdorf R,Li PF,Dietz R.Signaling pathways in reactive oxygen species-induced cardiomyocyte apoptosis[J].Circulation,1999,99(22):2934.
  • 7Palojoki E,Saraste A,Eriksson A,et al.Cardiomyocyte apoptosis and ventricular remodeling after myocardial infarction in rats[J].Am J Physiol Heart Circ Physiol,2001,280(6):H2726-H2731.
  • 8Takahashi T,Morita K,Akagi R,et,al.Heme oxygenase-1:a novel therapeutic target in oxidative tissue injuries.Curr Med Chem[J].2004,11(12):1545.
  • 9Fang J,Akaike T,Maeda H.Antiapoptotic role of heme oxygenase (HO) and the potential of HO as a target in anticancer treatment[J].Apoptosis.2004,9(1):27.

二级参考文献2

  • 1Hari S. Sharma,Dipak K. Das,Pieter D. Verdouw. Enhanced expression and localization of heme oxygenase-1 during recovery phase of porcine stunned myocardium[J] 1999,Molecular and Cellular Biochemistry(1-2):133~139
  • 2T. Asakawa,S. Matsushita. Thiobarbituric acid test for detecting lipid peroxides[J] 1979,Lipids(4):401~406

共引文献12

同被引文献94

  • 1王军民,张文敏.银杏酮酯滴丸联合替格瑞洛对冠心病心绞痛患者心绞痛发作情况及炎症反应的影响[J].反射疗法与康复医学,2022(2):66-69. 被引量:3
  • 2周辉,张安平,陈敏珠,徐叔云,汪国良.白芍总甙对应激大鼠下丘脑一垂体一肾上腺轴和免疫功能的调节[J].中国药理学通报,1994,10(6):429-432. 被引量:27
  • 3鄢顺琴,凤良元,丁荣光.金钱白花蛇抗炎作用的实验研究[J].中药材,1994,17(12):29-30. 被引量:6
  • 4Shao ZH, Xie JT, Vanden Hock TL, et al. Antioxidant effects of American ginseng berry extract in cardiomyocytes exposed to acute oxidant stress [ J]. Biochimica et Biophysica Acta, 2004, 1670 (3): 165- 171.
  • 5Jiang HK, Qiu GR, Li - Ling J, et al. Reduced ACTC1 expression might play a role in the onset of congenital heart disease by inducing cardiomyocyte apoptosis [ J]. Circ J, 2010, 74 (11): 2410 -2418.
  • 6Yang J, Liu X, Bhalla K, et al. Prevention of apoptosis by Bcl -2: release of cytochrome c from mitochondria blocked [ J]. Science, 1997, 275 (5303): 1129 -1132.
  • 7Jin YC, Kim W, Ha YM, et al. Propofol limits rat myocardial ischemia and reperfusion injury with an associated reduction in apoptotic cell death in vivo [J]. Vascul Pharmacol, 2009, 50 (1 -2): 71 -77.
  • 8Wu LX,Gu XF, Zhu YC, et al. Protective effects of novel single compound, Hirsutine on hypoxie neonatal rat cardiomyocytes [J]. Ear J Pharmacol, 2011, 650 (1): 290-297.
  • 9DcBiasi RL, Robinson BA, Loser JS, et al. Critical role for death - receptor mediated apoptotic signaling in viral myocarditis [J]. J Card Fail, 2010, 16 (11): 901-910.
  • 10Nunez G, Benedict MA, Hu Y, et al. Caspases:the protcas of the apoptotic pathway [ J ]. Oncogenc, 1998, 17 ( 25 ) : 3237 - 3245.

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